Mn-doped cerium dioxide nanozyme mediates ROS homeostasis and hormone metabolic network to promote wheat germination under low-temperature conditions

文献类型： 外文期刊

第一作者： Wu, Yixin

作者： Wu, Yixin;Xu, Shen;Sun, Mengqing;Liu, Lizhu;Shi, Gaoling;Gao, Yan;Wu, Yixin;Xu, Shen;Sun, Mengqing;Liu, Lizhu;Shi, Gaoling;Gao, Yan;Wei, Hui;Muhammad, Faheem;Muhammad, Faheem

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关键词： Wheat; Nanozyme; Seed priming; Cold tolerance; Hormone metabolic

期刊名称：CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE （ 影响因子：5.2； 五年影响因子：6.1 ）

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年卷期： 2025 年 12 卷 1 期

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收录情况： SCI

摘要： BackgroundNanozymes are a class of nanocatalytic materials that mimic the functions of natural enzymes. Their enzyme-like properties enable the catalytic scavenging of excess reactive oxygen species (ROS) generated in plants under abiotic stress, thereby alleviating oxidative stress. Research on nanozymes' role and related mechanisms in alleviating low-temperature stress in crops is still unclear. Therefore, developing nanozymes that enhance early stage cold tolerance in crops is critical for maintaining agricultural production and global food security.ResultsWe synthesized a nanozyme with catalase (CAT)-like activity, manganese-doped cerium oxide (MCNPs) nanoparticles. This study demonstrates that priming with MCNPs significantly accelerated wheat germination under cold stress, increasing the germination index by 7.1% and seedling biomass by 6.2-17.2% compared to hydropriming. Through SP-ICP-MS analysis, we confirmed that MCNPs can enter the seed. We also found that the catalase-like activity of MCNPs synergistically enhanced endogenous antioxidant enzymes (CAT and superoxide dismutase) to effectively eliminate excessive ROS in wheat seeds. Further analysis using LC-MS and qPCR showed that this ROS homeostasis influenced hormone metabolism by regulating the expression of genes involved in the hormone metabolic network, elevating growth-promoting hormones (gibberellin and ethylene) by 25.5-27.2% while suppressing stress-responsive hormones (jasmonic acid and abscisic acid). Subsequent activation of the gibberellin-responsive transcription factor TaGAMYB up-regulated amylase genes, boosting beta-amylase activity by 17.1-18.5% and accelerating starch hydrolysis into reducing sugars, collectively enhancing low-temperature germination.ConclusionsMCNP priming significantly alleviated the inhibitory effects of low temperature on wheat seed germination by coordinately regulating the processes of "ROS homeostasis," "hormone metabolism," and "starch hydrolysis," offering a promising strategy for enhancing plant cold tolerance and maintaining food security in the face of climate change.

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