Revealing microbial diversity in buffalo milk with high somatic cell counts: implications for mastitis diagnosis and treatment

文献类型： 外文期刊

第一作者： Jiang, Hancai

作者： Jiang, Hancai;Xu, Jiayin;Xu, Xiaoxian;Qin, Chaobin;Miao, Wenhao;Li, Ling;Song, Xinhui;Cui, Kuiqing;Li, Zhipeng;Wei, Jue;Liu, Jinfeng;Li, Ling;Liu, Qingyou;Cui, Kuiqing

作者机构：

关键词： Mastitis; Buffalo; 16S rDNA sequencing; Pathogens; Lactation; ELISA

期刊名称：VETERINARY RESEARCH COMMUNICATIONS （ 影响因子：2.0； 五年影响因子：2.1 ）

ISSN： 0165-7380

年卷期： 2024 年 48 卷 4 期

页码：

收录情况： SCI

摘要： Mastitis is one of the most serious diseases that threatens the health of dairy animals. The somatic cell count (SCC) in milk is widely used to monitor mastitis. This study aimed to reveal the diversity of microorganisms in buffalo milk with high somatic cell count (SCC >= 3 x 105 cells/mL, n = 30) and low somatic cell count (SCC <= 5 x 104 cells/mL, n = 10), and identify the dominant bacteria that cause mastitis in a local buffalo farm. We also investigated the potential method to treat bacterial mastitis. The V3-V4 region of 16 S rDNA was sequenced. Results showed that, compared to the milk with low SCC, the high SCC samples showed lower microbial diversity, but a high abundance of bacteria and operational taxonomic units (OTUs). By in vitro isolation and culture, Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae were found to be the leading pathogens, which is consistent with the 16 S rDNA sequencing data. We further isolated 3 of the main pathogens and established a pathogen detection method based on ELISA. In addition, the antibacterial effects of 10 antimicrobials and 15 Chinese herbal extracts were also investigated. Results showed that the microbial has developed tolerance to several of the antimicrobials. While the water extracts of Chinese herbal medicine such as Galla Chinensis, Coptis chinensis Franch, Terminalia chebula Retz, and Sanguisorba officinalis L can effectively inhibit the growth of main pathogens. This study provides novel insight into the microbial diversity in buffalo milk and a reference for the prevention, diagnosis, and treatment of mastitis.

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