The new CFEM protein CgCsa required for Fe 3+homeostasis regulates the growth, development, and pathogenicity of Colletotrichum gloeosporioides

文献类型： 外文期刊

第一作者： Liu, Sizhen

作者： Liu, Sizhen;Bu, Zhigang;Zhu, Yonghua;Liu, Sizhen;Zhang, Xin;Chen, Yue;Sun, Qianlong;Wu, Fei;Guo, Sheng;Tan, Xinqiu;Liu, Sizhen;Zhang, Xin;Chen, Yue;Sun, Qianlong;Wu, Fei;Guo, Sheng;Tan, Xinqiu;Tan, Xinqiu

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关键词： Colletotrichum gloeosporioides; CgCsa; CFEM; Pathogenicity; Iron

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：7.7； 五年影响因子：7.7 ）

ISSN： 0141-8130

年卷期： 2024 年 274 卷

页码：

收录情况： SCI

摘要： Secreted common fungal extracellular membrane (CFEM) domain proteins have been implicated in multiple biological functions in fungi. However, it is still largely unknown whether the ferric iron (Fe 3 + ), as an important trace element, was involved with the biological function of CFEM proteins. In this study, a new CFEM protein CgCsa, with high expression levels at the early inoculation stage on peppers by Colletotrichum gloeosporioides was investigated. Deletion of the targeted gene CgCsa revealed multiple biological roles in hyphal growth restriction, highly reduced conidial yield, delayed conidial germination, abnormal appressorium with elongated bud tubes, and significantly reduced virulence of C. gloeosporioides . Moreover, in CgCsa mutants, the expression levels of four cell wall synthesis -related genes were downregulated, and cell membrane permeability and electrical conductivity were increased. Compared to the wild -type, the CgCsa mutants downregulated expressions of iron transport -related genes, in addition, its three-dimensional structure was capable binding with iron. Increase in the Fe 3 + concentration in the culture medium partially recovered the functions of Delta CgCsa mutant. This is probably the first report to show the association between CgCsa and iron homeostasis in C. gloeosporioides . The results suggest an alternative pathway for controlling plant fungal diseases by deplete their trace elements.

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