Preparation and application of porcine broadly neutralizing monoclonal antibodies in an immunoassay for efficiently detecting neutralizing antibodies against foot-and-mouth disease virus serotype O

文献类型： 外文期刊

第一作者： Cao, Yimei

作者： Cao, Yimei;Li, Fengjuan;Xing, Xiangchuan;Zhang, Huiyan;Zhao, Qiongqiong;Sun, Pu;Fu, Yuanfang;Li, Pinghua;Ma, Xueqing;Zhang, Jing;Zhao, Zhixun;Yuan, Hong;Wang, Jian;Wang, Tao;Bao, Huifang;Bai, Xingwen;Li, Dong;Zhang, Qiang;Li, Kun;Lu, Zengjun

作者机构：

关键词： foot-and-mouth disease virus; porcine broadly neutralizing monoclonal antibody; competitive ELISA; neutralizing antibody; serotype O

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.8； 五年影响因子：4.1 ）

ISSN：

年卷期： 2025 年 13 卷 2 期

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收录情况： SCI

摘要： Neutralizing antibodies provide vital protection against foot-and-mouth disease virus (FMDV). The virus neutralization test (VNT) is a gold standard method for the detection of neutralizing antibodies. However, its application is limited due to the requirement for live virus and unsuitability for large-scale serological surveillance. In this study, a porcine broadly neutralizing monoclonal antibody (PO18-10) against FMDV was obtained from the heterologous sequentially vaccinated pig using single-B-cell antibody technology. A competitive enzyme-linked immunosorbent assay (C-ELISA) for detecting neutralizing antibodies against FMDV serotype O was developed using biotinylated PO18-10 as a detector antibody. The sensitivity and specificity of the assay were 100% and 99.55%, respectively, and the positive/negative coincidence rate with VNT was 94%, suggesting that C-ELISA based on natural host-derived monoclonal antibody (mAb) could be a promising tool to detect neutralizing antibodies against FMDV serotype O and evaluate the vaccine efficacy.

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