SfREPAT38, a pathogen response gene (REPAT), is involved in immune response of Spodoptera frugiperda larvae through mediating Toll signalling pathway
文献类型: 外文期刊
第一作者: Wang, Yuxue
作者: Wang, Yuxue;Mbiza, Natasha Isabel Tanatsiwa;Liu, Ting;Wang, Yi;Zhang, Yi;Luo, Xincheng;Chu, Longyan;Li, Jianping;Yang, Yazhen;Wang, Xiangping;Zhang, Jianmin;Yu, Yonghao
作者机构:
关键词: Bacillus thuringiensis; RNA interference; SfREPAT38; Spodoptera frugiperda; Toll signalling pathway
期刊名称:INSECT MOLECULAR BIOLOGY ( 影响因子:2.6; 五年影响因子:2.9 )
ISSN: 0962-1075
年卷期: 2024 年
页码:
收录情况: SCI
摘要: REPAT (response to pathogen) is an immune-associated gene family that plays important roles in insect immune response to pathogens. Although nine REPAT genes have been identified in Spodoptera frugiperda (Lepidoptera: Noctuidae) currently, their functions and mechanisms in the immune response to pathogens still remain unclear. Therefore, SfREPAT38, a pathogen response gene (REPAT) of S. frugiperda, was characterised and its function was analysed. The results showed that SfREPAT38 contains a signal peptide and a transcription activator MBF2 (multi-protein bridging factor 2) domain. Quantitative real-time polymerase chain reaction analysis showed that SfREPAT38 was highly expressed in the sixth-instar larvae (L6) and was the highest in expression in the midgut of L6. We found that the expression of SfREPAT38 could be activated by challenge with four microbial pathogens (Bacillus thuringiensis, Metarhizium anisopliae, Spodoptera exigua nuclearpolyhedrosis and Escherichia coli), except 12 h after E. coli infection. Furthermore, the SfREPAT38 expression levels significantly decreased at 24, 48 and 72 h after SfREPAT38 dsRNA injection or feeding. Feeding with SfREPAT38 dsRNA significantly decreased the weight gain of S. frugiperda, and continuous feeding led to the death of S. frugiperda larvae from the fourth day. Moreover, SfREPAT38 dsRNA injection resulted in a significant decrease of weight gain on the fifth day. Silencing SfREPAT38 gene down-regulated the expression levels of immune genes belonging to the Toll pathway, including SPZ, Myd88, DIF, Cactus, Pell and Toll18W. After treatment with SfREPAT38 dsRNA, S. frugiperda became extremely sensitive to the B. thuringiensis infection, and the survival rate dramatically increased, with 100% mortality by the eighth day. The weight of S. frugiperda larvae was also significantly lower than that of the control groups from the second day onwards. In addition, the genes involved in the Toll signalling pathway and a few antibacterial peptide related genes were down-regulated after treatment. These results showed that SfREPAT38 is involved in the immune response of S. frugiperda larvae through mediating Toll signalling pathway.
分类号:
- 相关文献
作者其他论文 更多>>
-
Extreme drought impacts have been underestimated in grasslands and shrublands globally
作者:Smith, Melinda D.;Knapp, Alan K.;Ohlert, Timothy;Smith, Melinda D.;Knapp, Alan K.;Wilkins, Kate D.;Holdrege, Martin C.;Beard, Karen H.;Kulmatiski, Andrew;Holdrege, Martin C.;Beard, Karen H.;Kulmatiski, Andrew;Wilfahrt, Peter;Anderson, Maggie;Borer, Elizabeth T.;Isbell, Forest;Seabloom, Eric W.;Collins, Scott L.;Sala, Osvaldo E.;Jordan, Samuel;Dukes, Jeffrey S.;Phillips, Richard P.;Beier, Claus;Yahdjian, Laura;Biancari, Lucio;Bondaruk, Viviana Florencia;Onatibia, Gaston R.;Pineiro-Guerra, Juan Manuel;Gherardi, Laureano A.;Larsen, Klaus Steenberg;Fraser, Lauchlan H.;Jentsch, Anke;Niu, Yujie;Loik, Michael E.;Maestre, Fernando T.;Valdecantos, Alejandro;Maestre, Fernando T.;Gozalo, Beatriz;Maturano-Ruiz, Adrian;Valdecantos, Alejandro;Power, Sally A.;Chieppa, Jeff;Nielsen, Uffe N.;Yu, Qiang;Felton, Andrew J.;Munson, Seth M.;Luo, Yiqi;Bauerle, Taryn;Abdoli, Hamed;Abedi, Mehdi;Bahalkeh, Khadijeh;Alados, Concepcion L.;Castillo-Garcia, Miguel;Pueyo, Yolanda;Alberti, Juan;Bruschetti, Carlos Martin;Cardoni, Augusto;Daleo, Pedro;Iribarne, Oscar;Alon, Moshe;Cohen, Ofer;Sternberg, Marcelo;An, Hui;Anacker, Brian;Auge, Harald;Auge, Harald;Eisenhauer, Nico;Hoss, Daniela;Roscher, Christiane;Sunnemann, Marie;Bachle, Seton;Bachle, Seton;Bahn, Michael;Ingrisch, Johannes;Batbaatar, Amgaa;Cahill, James F., Jr.;Batbaatar, Amgaa;Bork, Edward W.;Carlyle, Cameron N.;Behn, Kai;Beil, Ilka;Kreyling, Juergen;Malyshev, Andrey V.;Blindow, Irmgard;Dahlke, Sven;Byrne, Kerry M.;Calvo, Dianela A.;Leder, Cintia V.;Peter, Guadalupe;Carbognani, Michele;Forte, T'ai G. W.;Petraglia, Alessandro;Chang, Scott X.;Cianciaruso, Marcus V.;Guidoni-Martins, Karlo G.;Nobre, Paola;Cordeiro, Amanda L.;Cusack, Daniela F.;Dietterich, Lee H.;D'Antonio, Carla M.;Dietterich, Lee H.;Doherty, Tim S.;Greenville, Aaron C.;Wardle, Glenda M.;Dubbert, Maren;Ebeling, Anne;Eisenhauer, Nico;Fischer, Felicia M.;Hoss, Daniela;Sunnemann, Marie;Hoss, Daniela;Pillar, Valerio D.;Fischer, Felicia M.;Gebauer, Tobias;Anacker, Brian;Haugum, Siri Vatso;Vandvik, Vigdis;Hautier, Yann;Hefting, Mariet;Henry, Hugh A. L.;Johnson, Yari;Kelly, Eugene F.;Kimmel, Kaitlin;Kroel-Dulay, Gyorgy;Orban, Ildiko;Kroel-Dulay, Gyorgy;Orban, Ildiko;Kubert, Angelika;Werner, Christiane;Lamb, Eric G.;Larson, Julie;O'Connor, Rory C.;Lawson, Jason;Plowes, Robert M.;Linstadter, Anja;Orban, Ildiko;Liu, Jielin;Liu, Shirong;Longo, Grisel;Loydi, Alejandro;Rodriguez, Dana Aylen;Scarfo, Maria Cecilia;Luan, Junwei;Wang, Yi;Luan, Junwei;Wang, Yi;Lubbe, Frederick Curtis;Wiehl, Georg;Mackie-Haas, Kathleen;Stampfi, Andreas;Merchant, Thomas;Metcalfe, Daniel B.;Mori, Akira S.;Mori, Akira S.;Mudongo, Edwin;Newman, Gregory S.;Souza, Lara;Nimmo, Dale;Ogaya, Roma;Penuelas, Josep;Osborne, Brooke;Otfinowski, Rafael;Partel, Meelis;Pineiro-Guerra, Juan Manuel;Peri, Pablo L.;Picon-Cochard, Catherine;Pineiro-Guerra, Juan Manuel;Ploughe, Laura W.;Portales-Reyes, Cristy;Reed, Sasha C.;Ritchie, Euan G.;Roscher, Christiane;Sanchez, Ana M.;Santo, Braulio A.;Shi, Baoku;Sun, Wei;Souza, Lara;Standish, Rachel J.;Zeiter, Michaela;Stampfi, Andreas;Zeiter, Michaela;Standish, Rachel J.;Tedder, Michelle;Thorvaldsen, Pal;Tian, Dashuan;Zhu, Juntao;Zong, Ning;Tielborger, Katja;van den Brink, Liesbeth;Vankoughnett, Mathew R.;Velle, Liv Guri;Wang, Changhui;Wei, Cunzheng;Williams, Jennifer L.;Wolf, Amelia A.;Zhang, Fawei;Zuo, Xiaoan;Zuo, Xiaoan;Gebauer, Tobias;Ploughe, Laura W.
关键词:climate extreme; Drought-Net; International Drought Experiment; productivity
-
Development and Characterization of an Antioxidant and Antimicrobial Film Composited by Hydroxyethyl Cellulose and Sulfated Rice Bran Polysaccharides for Food Packaging
作者:Chen, Bing-Jie;Wang, Xiao;Liu, Hong-Ru;Zhang, Yi;Wang, Chun-Fang;Liu, Chen-Xia;Qiao, Yong-Jin;Liu, Gui-Ge
关键词:hydroxyethyl cellulose; sulfated rice bran polysaccharides; film; antioxidant; bacteriostatic properties
-
Response of Interaction between Aboveground and Belowground Herbivorous to Corn Development
作者:Liu, Jinwen;Li, Hong;Zhang, Jinhua;Li, Jianping;Yan, Xiujuan
关键词:Aboveground herbivores; Belowground herbivores; Plant development; Community structure; Interactions
-
A sensor based on Fe/B/N codoped hierarchical porous carbon spheres that amplify the aggregation-induced electrochemiluminescence of calcein for florfenicol trace detection
作者:Deng, Yan;Li, Shuhuai;Zhang, Lianming;Li, Jianping;Deng, Yan;Pang, Chaohai;Li, Shuhuai;Ma, Xionghui;Wu, Yuwei
关键词:Aggregation -induced; electrochemiluminescence; Porous carbon spheres; Calcein; Florfenicol; Sensor
-
A comparison of chemical composition, bioactive components and hypoglycemic activity of Stigma maydis obtained from different growing times
作者:Zhang, Yi;Pang, Jinxin;Yao, Lianmou;Wang, Chunfang;Chen, Bingjie;Qiao, Yongjin;Zhang, Yi;Pang, Jinxin;Yao, Lianmou;Wang, Chunfang;Chen, Bingjie;Hu, Yingxiong;Qiao, Yongjin;Zheng, Qi;Qiao, Yongjin
关键词:Stigma maydis; maturity stage; chemical composition; bioactive components; hyperglycemic activity
-
Precipitation and plant community-weighted mean traits determine total transpirable soil water in a desert grassland
作者:Chen, Juan;Song, Naiping;Chen, Juan;Song, Naiping;Wang, Xing;Meng, Chen;Zhang, Yi;Chen, Lin;Wang, Qixue;Lv, Hao;Chen, Juan;Song, Naiping;Wang, Xing;Meng, Chen;Zhang, Yi;Chen, Lin;Wang, Qixue;Lv, Hao;Song, Naiping;Wang, Xing;Meng, Chen;Zhang, Yi;Chen, Lin;Wang, Qixue;Lv, Hao;Wu, Xudong;Yu, Dian
关键词:Functional diversity; Selection effect; Ecosystem function; Total transpirable soil water; Desert steppe
-
A nonsynonymous mutation in BhLS, encoding an acyl-CoA N-acyltransferase leads to fruit and seed size variation in wax gourd (Benincasa hispida)
作者:Yan, Jinqiang;Xie, Dasen;Wang, Yi;Wang, Min;Yang, Songguang;Jiang, Biao;Chen, Lin;Cai, Jinsen;Liu, Wenrui;Cheng, Jiaowen
关键词: