Analysis of the Immunogenicity of African Swine Fever F317L Protein and Screening of T Cell Epitopes

文献类型： 外文期刊

第一作者： Huang, Ying

作者： Huang, Ying;Zhai, Wenzhu;Wang, Zhen;He, Yuheng;Tao, Chunhao;Chu, Yuanyuan;Pang, Zhongbao;Zhu, Hongfei;Jia, Hong;He, Yuheng;Chu, Yuanyuan

作者机构：

关键词： African swine fever virus; F317L protein; cellular immunity; humoral immunity; T cell epitope

期刊名称：ANIMALS （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 9 期

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收录情况： SCI

摘要： Simple Summary The African swine fever virus (ASFV) is a linear double-stranded DNA virus characterized by a complex immune escape mechanism that has caused substantial economic losses to the global swine industry. An in-depth study of ASFV has revealed that screening for protective antigens and activating cellular immunity is essential for the development of ASF vaccines. In this study, we sought to examine the immunogenicity of the F317L protein of ASFV in mice while also identifying potential active T-cell epitopes in order to provide a basis for the functional study of the F317L protein, in addition to a reference for the subsequent development of ASF vaccines.Abstract The African swine fever virus (ASFV) encodes numerous proteins characterized by complex immune escape mechanisms. At present, the structure and function of these proteins, including the F317L protein, have yet to be fully elucidated. In this study, we examined the immunogenicity of the F317L protein. Mice were subcutaneously immunized with the F317L protein using initial and subsequent booster doses, and, at the 28th day post-treatment, we assessed the humoral and cellular immune responses of mice. The F317L protein stimulated production of specific antibodies and activated humoral immune responses. In addition, F317L stimulated the production of large amounts of IFN-gamma by splenic lymphocytes, thereby activating cellular immune responses. Using informatics technology, we predicted and synthesized 29 F317L protein T cell epitopes, which were screened using IFN-gamma ELISpot. Among these, the F25 (246SRRSLVNPWT255) peptide was identified as having a stronger stimulatory effect than the full-length protein. Collectively, our findings revealed that the ASFV F317L protein can stimulate both strong humoral and cellular immunity in mice, and that the F25 (246SRRSLVNPWT255) peptide may be a potential active T cell epitope. These findings will provide a reference for further in-depth studies of the F317L protein and screening of antigenic epitopes.

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