N-Glycan Profiles of Neuraminidase from Avian Influenza Viruses

文献类型： 外文期刊

第一作者： Chen, Wentian

作者： Chen, Wentian;Ma, Tianran;Liu, Sinuo;Zhong, Yaogang;Yu, Hanjie;Shu, Jian;Li, Zheng;Wang, Xiurong

作者机构：

关键词： avian influenza virus; neuraminidase; N-glycan profiles; lecin microarrays; MALDI-TOF/TOF-MS

期刊名称：VIRUSES-BASEL （ 影响因子：4.7； 五年影响因子：4.8 ）

ISSN：

年卷期： 2024 年 16 卷 2 期

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收录情况： SCI

摘要： The cleavage of sialic acids by neuraminidase (NA) facilitates the spread of influenza A virus (IV) descendants. Understanding the enzymatic activity of NA aids research into the transmission of IVs. An effective method for purifying NA was developed using p-aminophenyloxamic acid-modified functionalized hydroxylated magnetic particles (AAMPs), and from 0.299 to 0.401 mg of NA from eight IV strains was isolated by 1 mg AAMP. A combination of lectin microarrays and MALDI-TOF/TOF-MS was employed to investigate the N-glycans of isolated NAs. We found that more than 20 N-glycans were identified, and 16 glycan peaks were identical in the strains derived from chicken embryo cultivation. Multi-antennae, bisected, or core-fucosylated N-glycans are common in all the NAs. The terminal residues of N-glycans are predominantly composed of galactose and N-acetylglucosamine residues. Meanwhile, sialic acid residue was uncommon in these N-glycans. Further computational docking analysis predicted the interaction mechanism between NA and p-aminophenyloxamic acid.

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