Expression of ASFV p17 in CHO cells and identification of one novel epitope using a monoclonal antibody

文献类型： 外文期刊

第一作者： Li, Liwei

作者： Li, Liwei;Qiao, Sina;Wang, Shumao;Liu, Jiachen;Zhou, Yanjun;Li, Guoxin;Jiang, Yifeng;Liu, Changlong;Tong, Guangzhi;Tong, Wu;Gao, Fei;Qiao, Sina;Zhao, Kuan;Wang, Shumao;Tong, Wu;Gao, Fei;Tong, Wu;Gao, Fei

作者机构：

关键词： ASFV; P17; CHO cells; Monoclonal antibody; Epitope

期刊名称：VIRUS RESEARCH （ 影响因子：5.0； 五年影响因子：4.0 ）

ISSN： 0168-1702

年卷期： 2023 年 336 卷

页码：

收录情况： SCI

摘要： As a highly pathogenic large DNA virus, African swine fever virus (ASFV) has huge particles and numerous encoded proteins. At present, few of the existing studies on ASFV proteins have investigated the function of p17. Specific antibodies against p17 to promote the development of prevention techniques against African swine fever (ASF) are urgently needed. Herein, we successfully expressed ASFV p17 in CHO cells using a suspension culture system and generated a monoclonal antibody (mAb) against p17. The mAb recognized a novel linear epitope (8LLSHNLSTREGIK20) and exhibited specific reactivity, which was conducive to the identification of ectopically expressed p17, the recombinant porcine reproductive and respiratory syndrome virus expressing p17, and the ASFV-SY18. The epitope was conservative among genotype I and genotype II ASFV strains. Overall, the mAb against p17 revealed efficient detection and promising application prospects, making it a useful tool for future vaccine research on ASF. Determination of the conserved linear epitope of p17 would contribute to the in-depth exploration of the biological function of ASFV antigen protein.

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