A magnetic nanoparticle-based lateral flow immunochromatography assay for the rapid detection of fluoroquinolones in milk

文献类型： 外文期刊

第一作者： Liu, Chang

作者： Liu, Chang;Yang, Linyan;Zhang, Wei;Li, Daowen;Li, Liuan;Wang, Hongyu;Li, Cun;Ma, Yi

作者机构：

关键词： Magnetic nanoparticles; Lateral flow immunochromatography assay; Fluoroquinolones; Milk sample; Rapid detection

期刊名称：EUROPEAN FOOD RESEARCH AND TECHNOLOGY （ 影响因子：2.998； 五年影响因子：3.005 ）

ISSN： 1438-2377

年卷期： 2021 年 247 卷 10 期

页码：

收录情况： SCI

摘要： A magnetic nanoparticle (MNP)-based lateral flow immunochromatography assay (LFIA) was established to detect fluoroquinolones (FQs) in milk. This method is an improvement of traditional Au nanoparticle (AuNP)-based LFIA. Compared with AuNP-labeled antibody, the combination between MNPs and antibody is stronger and more stable, and the preparation process is convenient. In this study, the color intensity of MNPs-LFIA test strip was analyzed, which laid a research foundation for magnetic quantitative analysis and high sensitivity analysis of LFIA. The limits of detection (LODs) for 10 FQs in phosphate buffer (PB) solution ranged from 1 to 5 ng/mL, and in milk ranged from 2 to 20 ng/mL. The recovery in spiked milk sample ranged from 16.47 to 83.67%. The log functions of analyte concentration and T/C value were fitted into a linear equation. The specificity of the MNPs-LFIA is satisfactory, without cross-reaction with antibiotics from other chemical classes. The results of this method are consistent with those of ultra-performance liquid chromatography-mass spectrometry, indicating the MNPs-LFIA method is reliable and accurate. As far as we know, this is the first time that MNPs-LIFA has been applied to the detection of FQs in milk.

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