EtcPRS(Mut) as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondii

文献类型： 外文期刊

第一作者： Sun, Pei

作者： Sun, Pei;Liu, Jie;Chen, Linlin;Shi, Fangyun;Hao, Zhenkai;Suo, Jingxia;Suo, Xun;Liu, Xianyong;Sun, Pei;Liu, Jie;Chen, Linlin;Shi, Fangyun;Hao, Zhenkai;Suo, Jingxia;Suo, Xun;Liu, Xianyong;Zhang, Yuanyuan;Zhang, Yuanyuan;Wang, Chaoyue;Hu, Dandan;Tang, Xinming;Yu, Yonglan

作者机构：

期刊名称：ISCIENCE （ 影响因子：5.8； 五年影响因子：5.8 ）

ISSN：

年卷期： 2023 年 26 卷 4 期

页码：

收录情况： SCI

摘要： The control of coccidiosis, causing huge economic losses in the poultry industry, is facing the stagnation of the development of new drugs and the emergence of drug resistance. Thus, the priority for coccidiosis control is to decipher the effect mechanisms and resistance mechanisms of anticoccidial drugs. In this study, we mined and validated a molecular marker for halofuginone resistance in Eimeria tenella through forward and reverse genetic approaches. We screened whole-genome sequencing data and detected point mutations in the ETH2_1020900 gene (encoding prolyl-tRNA synthetase, PRS). Then, we introduced this mutated gene into E. tenella and Toxoplasma gondii and validated that overexpression of this mutated gene confers resistance to halofuginone in vivo and in vitro. These results together show that mutations A1852G and A1854G on the ETH2_ 1020900 gene are pivotal to halofuginone resistance in E. tenella, encouraging the exploration of mechanisms of drug resistance against other anticoccidial drugs in eimerian parasites.

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