Development of reverse-transcription loop-mediated isothermal amplification for the detection of infectious bursal disease virus

文献类型： 外文期刊

第一作者： Xu, Jiangtao

作者： Xu, Jiangtao;Yin, Yanbo;Xu, Shouzhen;Wang, Jianlin;Liu, Xingcai;Zhang, Zhenmei;Cui, Shangjin;Guo, Yanyan;Li, Jida;Han, Limin

作者机构：

关键词： Infection;Methods and Techniques

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To establish a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of infectious bursal disease virus (IBDV), four primers specific to six regions of the VP3 gene were designed; the VP3 region was selected because it is a conserved part of the IBDV genome. After amplification in an isothermal water bath for 70 min, samples containing IBDV generated the expected ladder-like products while other viruses generated no product. The sensitivity and specificity of the RT-LAMP assay were evaluated by comparison with reverse-transcription polymerase chain reaction (RT-PCR) and virus isolation. The assay was significantly more sensitive than normal gel-based RT-PCR Because it is specific and simple, the RT-LAMP assay can be widely applied in clinical laboratories for rapid detection of IBVD.

分类号： R37

  • 相关文献

[1]An improved assay for detection of Acidovorax citrulli in watermelon and melon seed. Zhao, T.,Feng, J.,Li, J.,Randhawa, P..

[2]Detection of red-spotted grouper nervous necrosis virus by loop-mediated isothermal amplification. Xu, Hai-Dong,Feng, Juan,Guo, Zhi-Xun,Ou, You-Jun,Wang, Jiang-Yong,Xu, Hai-Dong.

[3]Development and validation of a SIgA-ELISA for the detection of Mycoplasma hyopneumoniae infection. Feng, Zhi-Xin,Shao, Guo-Qing,Liu, Mao-Jun,Wang, Hai-Yan,Gan, Yuan,Wu, Xu-Su,Feng, Zhi-Xin,Shao, Guo-Qing,Liu, Mao-Jun,Wang, Hai-Yan,Gan, Yuan,Wu, Xu-Su,Feng, Zhi-Xin,Shao, Guo-Qing,Liu, Mao-Jun,Wang, Hai-Yan,Gan, Yuan,Wu, Xu-Su.

[4]Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus. Sun, Yuan,Liu, Da-Fei,Wang, Yu-Fei,Liang, Bing-Bing,Cheng, Dan,Li, Na,Qi, Qiao-Fen,Zhu, Qing-Hu,Qiu, Hua-Ji.

[5]A TaqMan real-time PCR assay for quantifying white spot syndrome virus (WSSV) infections in wild broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis. Meng, Xian-Hong,Jang, In-Kwon,Meng, Xian-Hong,Seo, Hyung-Chul,Cho, Yeong-Rok,Kim, Bong-Rae,Ayyaru, Gopalakannan,Kim, Jong-Sheek.

[6]Determination of Mepiquat Chloride Residues in Cotton and Soil by Liquid Chromatography/Mass Spectrometry. Dong, Feng-Shou,Li, Shuo,Zheng, Yong-Quan.

[7]Influence of docosahexaenoic acid on the concentration of fatty acids and volatile fatty acids in rumen fluid analysed by a rumen-simulation techniques. Liu, L.,Wang, J. Q.,Bu, D. P.,Liu, S. J.,Liang, S.,Liu, K. L.,Wei, H. Y.,Zhou, L. Y..

[8]Ri-mediated Transformation of Glycyrrhiza uralensis with a Squalene Synthase Gene (GuSQS1) for Production of Glycyrrhizin. Lu, Hong-Yu,Zhang, Hai-Chao,Gao, Shan-Lin,Liu, Jing-Mei,Yin, Tao.

[9]Molecular Cloning and Expression of cDNA Encoding the Cysteine Proteinase Inhibitor from Upland Cotton. Jiang, Ming-feng,Li, Sheng-wei,Chen, Min,Li, Sheng-wei,Chen, Min,Cai, Ying-fan,Xie, Yong-fang,Li, Biao,Sun, Quan,Jiang, Huai-zhong,Pan, Zheng,Gao, Yun-ling,Yuan, You-Lu,Shi, Yu-zheng.

[10]The development and evaluation of a loop-mediated isothermal amplification (LAMP) method for detection of Babesia spp. infective to sheep and goats in China. Guan, Guiquan,Luo, Jianxun,Liu, Zhijie,Gao, Jinliang,Ma, Miling,Liu, Aihong,Dang, Zhisheng,Liu, Junlong,Ren, Qiaoyun,Jin, Yurong,Yin, Hong,Guan, Guiquan,Chauvin, Alain,Moreau, Emmanuelle,Inoue, Noboru,Thekisoe, Oriel M. M.,Sugimoto, Chihiro.

[11]Field-Testing of Synthetic Herbivore-Induced Plant Volatiles as Attractants for Beneficial Insects. Zhang, Yongjun,Wu, Kongming,Guo, Yu Yuan,Yu, Huilin.

[12]Identification of Heterodera glycines using PCR with sequence characterised amplified region (SCAR) primers. Peng, Deliang,Ou, Shiqi,Liu, Xuemin,Li, Yu,Moens, Maurice,Moens, Maurice.

[13]Development of SNP markers and haplotype analysis of the candidate gene for rhg1, which confers resistance to soybean cyst nematode in soybean. Li, Ying-Hui,Zhang, Chen,Ma, Zulu,Liu, Zhang-Xiong,Nan, Hai-Yang,Chang, Ru-Zhen,Qiu, Li-Juan,Gao, Zhong-Shan,Smulders, Marinus Johannes Maria,Ma, Zulu.

[14]Cloning and characterization of a novel cysteine protease gene (HbCP1) from Hevea brasiliensis. Peng, Shi-Qing,Zhu, Jia-Hong,Li, Hui-Liang,Tian, Wei-Min.

[15]Application of comparative genomics in chicken gene mapping. Tian, Yong,Lu, Lizhi,Wang, Deqian,Li, Guoqin,Yuan, Aiping,Yuan, Qingyan,Tian, Yong,Fu, Yan,Zhao, Jin.

[16]Development of rapid immunoassays for the detection of ractopamine in swine urine. Li, X.,Zhang, G.,Deng, R.,Yang, Y.,Liu, Q.,Xiao, Z.,Yang, J.,Xing, G.,Zhao, D.,Cai, S..

[17]Cloning and Expression of the Porcine MEF2a Gene. Zhang, Dong-jie,Liu, Di,Yang, Guo-wei,Gao, Zhi-sheng.

[18]A backward propagation neural network for predicting daily transpiration of poplar. Meijun, Yan,Peiling, Yang,Shumei, Ren,Yuanpei, Luo,Tingwu, Xu.

[19]Successful micropropagation protocol of Piper methysticum. Zhang, Z.,Zhao, L.,Chen, X.,Zheng, X..

[20]Detection of influenza viral gene in European starlings and experimental infection. Qin, Zhuoming,Clements, Teresa,Wang, Leyi,Khatri, Mahesh,Pillai, Smitha P. S.,LeJeune, Jeffrey T.,Lee, Chang-Won,Qin, Zhuoming,Zhang, Yan,LeJeune, Jeffrey T.,Lee, Chang-Won. 2011

作者其他论文 更多>>

微信小程序