Enhanced production of a thermostable mannanase by immobilized cells of Bacillus subtilis on various membranes
文献类型: 外文期刊
第一作者: Wu, Aimin
作者: Wu, Aimin;Jiang, Zhengqiang;Tang, Luo;Yan, Qiaojuan;Bo, Shi
作者机构:
关键词: beta-MANNOSIDASES;BACILLUS SUBTILIS;BACILLUS;GLYCOSIDASES;IMMOBILIZATION;IMMOBILIZED CELLS
期刊名称:WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY ( 影响因子:3.312; 五年影响因子:3.58 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Cells of the thermophilic Bacillus subtilis WY34 were immobilized on various formaldehyde-activated polymer membranes and the immobilized cells were used for the production of thermostable mannanase in flasks. The results showed that polyethersulfone membranes (PES) and nylon-6 membranes were the most suitable supports for cell immobilization to produce the mannanase. Moreover, PES and nylon-6 membranes immobilized cells provided 1.78- and 1.74-fold higher mannanase activity compared to the control after 4 days of cultivation, respectively. The immobilized cells on PES and nylon-6 membranes had good stability and retained 131.5 and 114.3% of ability of enzyme production even after six cycles of repeated batch fermentation, respectively. Active cell growth was observed by scanning electron microscopy (SEM) after 16 days (four cycles) repeated batch cultivation. Therefore, the membrane-immobilized cells of B. subtilis WY34 can be proposed as an effective biocatalyst for repeated usage for production of the thermostable mannanase.
分类号: Q93
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