Purification and characterization of a glycoprotein elicitor from Alternaria tenuissima

文献类型： 外文期刊

第一作者： Qiu, Dewen

作者： Qiu, Dewen;Zeng, Hongmei;Yuan, Jingjing;Mao, Jianjun

作者机构：

关键词： Alternaria tenuissima;Glycoprotein elicitor;Purification;Character;Inducing plant resistance

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

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年卷期：

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收录情况： SCI

摘要： Glycoprotein elicitor can induce plant resistance and become a potential agent for biological control of plant diseases. Here, a new glycoprotein elicitor was purified with the method of cold alcohol precipitation and anion exchange chromatography from the mycelium of Alternaria tenuissima strain JH505, which was identified on the basis of morphological features and sequence analysis of rDNA internal transcribed spacer. The protein showed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) stained with silver and appeared one main protein peak in HPLC. The apparent molecular weight of the purified protein was 66 kDa and isoelectric point was about 4.27. This protein was identified as glycoprotein by glycoprotein staining Kit. Anthrone-colorimetric assay and Coomassie blue G-250 staining showed that carbohydrate and protein content was in a ratio of 1.75. After deglycosylation by trifluoromethane-sulfonic acid, this glycoprotein showed two bands on the SDS-PAGE, and which means the glycoprotein may have at least two glycosylation sites. The glycoprotein induced tobacco resistance against tobacco mosaic virus and enhanced wheat seedling growth at 15pC. The glycoprotein elicitor provided an effective way of alternative strategies for plant disease control.

分类号： Q93

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