SAD, a Stearoyl-Acyl Carrier Protein Desaturase Highly Expressed in High-Oil Maize Inbred Lines

文献类型： 外文期刊

第一作者： Liu, Zh. J.

作者： Liu, Zh. J.;Yang, X. H.;Fu, Y.

作者机构：

关键词： high-oil maize; SAD; 2-DE; gas chromatography; real-time PCR

期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）

ISSN： 1021-4437

年卷期： 2009 年 56 卷 5 期

页码：

收录情况： SCI

摘要： As special maize with more than 6% oil concentration in the grain, high-oil maize has received increased interest recently. To date, little is known about the expressions of genes involved in fatty acid metabolism of high-oil maize. Stearoyl-acyl carrier protein desaturase (SAD) is a key enzyme that converts stearic acid to oleic acid. In this study, two-dimensional electrophoresis, gas chromatography, and real-time PCR were used to determine the expressions of SAD at three seed development stages in high-oil and normal maize inbred lines. SAD was significantly more abundantly expressed in high-oil maize than in normal maize, not only at the protein and mRNA levels, but also at the product level. These results suggested that a high expression of SAD may play an important role in increasing oil concentration in high-oil maize.

分类号： Q94

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