Gene transferability from transgenic Brassica napus L. to various subspecies and varieties of Brassica rapa
文献类型: 外文期刊
第一作者: Xiao, Ling
作者: Xiao, Ling;Yu, Deyue;Xiao, Ling;Lu, Changming;Bo, Huijie;Wu, Yuhua;Wu, Gang;Cao, Yinglong;Zhang, Bing
作者机构:
关键词: Transgenic Brassica napus;Brassica rapa subspecies;Crossability;Outcrossing rate;Fertilization process;Germination
期刊名称:TRANSGENIC RESEARCH ( 影响因子:2.788; 五年影响因子:2.377 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Gene transferability from transgenic rapeseed to various subspecies and varieties of Brassica rapa was assessed in this study. Artificial crossability was studied in 118 cultivars of 7 B. rapa subspecies and varieties with the transgenic rapeseed GT73 (Brassica napus) as the pollen donor. On average 5.7 seeds were obtained per pollination, with a range from 0.05 to 19.4. The heading type of B. rapa L. showed significantly higher crossability than non-heading types of B. rapa. The spontaneous outcrossing rate between B. rapa (female) and the transgenic rapeseed Ms8 x Rf3 (B. napus) (male) ranged from 0.039 to 0.406%, with an average of 0.19%. The fertilization process and the development of the hybrid seeds as shown by fluorescent staining techniques indicated that the number of adhered pollens on the stigma was reduced by 80%, the number of pollen tubes in the style was reduced by 2/3 and the fertilization time was delayed by over 20 h when pollinated with the transgenic rapeseed Ms8 x Rf3 in comparison with the bud self-pollination of B. rapa as control. About 10-70% of the interspecific hybrid embryos were aborted in the course of development. Some seeds looked cracked in mature pods, which showed germination abilities lower than 10%. The spontaneous outcrossing rates were much lower than the artificial crossability, and their survival fitness of the interspecific hybrid was very low, indicating that it should be possible to keep the adventitious presence of the off-plants under the allowed threshold, if proper measures are taken.
分类号: R394
- 相关文献
作者其他论文 更多>>
-
Fungal diversity notes 1717-1817: taxonomic and phylogenetic contributions on genera and species of fungal taxa
作者:Liu, Shi-Liang;Wang, Xue-Wei;Razaghi, Parisa;Raza, Mubashar;Cai, Lei;Jiang, Shuhua;Zhang, Chao;Zhou, Li-Wei;Wang, Xue-Wei;Li, Guo-Jie;Deng, Chun-Ying;Rossi, Walter;Leonardi, Marco;Liimatainen, Kare;Niskanen, Tuula;Kekki, Tapio;Smith, Matthew E.;Ammirati, Joe;Ammirati, Joe;Bojantchev, Dimitar;Abdel-Wahab, Mohamed A.;Zhang, Ming;Tian, Enjing;Bau, Tolgor;Lu, Yong-Zhong;Zhang, Jing-Yi;Ma, Jian;Zhang, Jing-Yi;Ma, Jian;Du, Tian-Ye;Peng, Xing-Can;Hyde, Kevin D.;Dutta, Arun Kumar;Acharya, Krishnendu;Saha, Rituparna;Tarafder, Entaj;Du, Tian-Ye;Du, Tian-Ye;Peng, Xing-Can;Hyde, Kevin D.;Xu, Jize;Kim, Ji Seon;Lim, Young Woon;Kim, Ji Seon;Lim, Young Woon;Gerlach, Alice;Zeng, Nian-Kai;Han, Yun-Xiao;Raza, Mubashar;Calabon, Mark S.;Jones, E. B. Gareth;Kumar, T. K. Arun;Krishnapriya, K.;Thomas, Anjitha;Kaliyaperumal, Malarvizhi;Kezo, Kezhocuyi;Gunaseelan, Sugantha;Singh, Sanjay Kumar;Singh, Paras Nath;Lagashetti, Ajay Chandrakant;Pawar, Kadambari Subhash;Rana, Shiwali;Zhang, Chao;Zhang, Huang;Qing, Yun;Peng, Xing-Can;Wen, Ting-Chi;Peng, Xing-Can;Wen, Ting-Chi;Ramirez, Natalia A.;Niveiro, Nicolas;Li, Mei-Xiang;Yang, Zhu L.;Wu, Gang;Li, Mei-Xiang;Yang, Zhu L.;Wu, Gang;Tennakoon, Danushka S.;Suwannarach, Nakarin;Kumla, Jaturong;Lumyong, Saisamorn;Tennakoon, Danushka S.;Suwannarach, Nakarin;Kumla, Jaturong;Lumyong, Saisamorn;Kuo, Chang-Hsin;da Silva, Tatiane M.;Bezerra, Jadson D. P.;Souza-Motta, Cristina M.;He, Gang;Ji, Xiao-Hong;Wannathes, Nopparat
关键词:95 new taxa; Six new records; Ascomycota; Basidiomycota
-
The impact of microplastics on sulfur REDOX processes in different soil types: A mechanism study
作者:Dong, Youming;Gao, Minling;Cai, Qiqi;Xiao, Ling;Chen, Zimin;Peng, Hongchang;Song, Zhengguo;Qiu, Weiwen;Liu, Qinghai
关键词:Polystyrene; Polyphenylene sulfide; Soil enzyme; Soil organic matter; Soil microorganism
-
The GmSTF1/2-GmBBX4 negative feedback loop acts downstream of blue-light photoreceptors to regulate isoflavonoid biosynthesis in soybean
作者:Song, Zhaoqing;Zhao, Fengyue;Chu, Li;Lin, Huan;Xiao, Yuntao;Fang, Zheng;Yu, Deyue;Gai, Junyi;Xu, Dongqing;Wang, Xuncheng;Dong, Jie;Lyu, Xiangguang;Liu, Bin
关键词:photoreceptor; light signaling; isoflavonoid; GmSTF; GmBBX4; soybean
-
The Development of a Series of Genomic DNA Reference Materials with Specific Copy Number Ratios for The Detection of Genetically Modified Maize DBN9936
作者:Li, Jun;Gao, Hongfei;Li, Yunjing;Zhai, Shanshan;Xiao, Fang;Wu, Gang;Wu, Yuhua
关键词:DBN9936; genomic DNA; certified reference material; duplex droplet digital PCR
-
Evaluation of reference genes for quantitative expression analysis in Mylabris sibirica (Coleoptera, Meloidae)
作者:Shen, Chen-Hui;Tang, Min;Li, Xiao-Fei;Zhu, Li;Wu, Gang;Yan, Xiao-Hong;Li, Wei;Deng, Pan;Zhai, Qing
关键词:Mylabris sibirica; reference gene; RT-qPCR; RefFinder; geNorm
-
Fabrication and characterization of thermal barrier coatings for internal combustion engines via suspension plasma spray with high solid loading
作者:Guo, Lei;Zhang, Bing;Guo, Lei;He, Qing;Liang, Likang;Liu, Mingguang
关键词:Thermal barrier coatings; Suspension plasma spraying; High solid loading; Parameters optimization; Feathery columnar structure; Thermal shock
-
Biotransformation of HBCDs by the microbial communities enriched from mangrove sediments
作者:Yu, Fei;Zhang, Bing;Liu, Yongjin;Luo, Wenqi;Chen, Haonan;Gao, Jun'na;Peng, Tao;Wang, Hui;Huang, Tongwang;Hu, Zhong;Ye, Xueying;Li, Jin;Xie, Qingyi
关键词:Microbial community; HBCDs; Biotransformation; Dehalogenase