Cloning, chromosomal localization, SNP detection and association analysis of the porcine IRS-1 gene

文献类型： 外文期刊

第一作者： Niu, P. -X.

作者： Niu, P. -X.;Huang, Z.;Li, C. -C.;Fan, B.;Liu, B.;Yu, M.;Zhao, S. -H.;Li, K.

作者机构：

关键词： Pig;IRS-1;Cloning;Mapping;Association analysis

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Insulin receptor substrate-1(IRS-1) gene is one member of the Insulin receptor substrate (IRS) gene family, which plays an important role in mediating the growth of skeletal muscle and the molecular metabolism of type 2 diabetes. Here, we cloned a 3,573 bp fragment of the partial CDS sequence of porcine IRS-1 gene by in silicon cloning strategy and RT-PCR method. The porcine IRS-1 gene was assigned to SSC15q25 by using IMpRH. Sequencing of PCR products from Duroc and Tibetan pig breeds identified one SNP in exon 1 of porcine IRS-1 gene (C3257A polymorphisms). Association analysis of genotypes with the growth traits, anatomy traits, meat quality traits and physiological biochemical indexes traits showed that different genotypes at locus 3,257 of IRS-1 have significant differences in carcass straight length in pigs (P = 0.0102 < 0.05).

分类号： Q7

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