ClSnRK2.3 negatively regulates watermelon fruit ripening and sugar accumulation

文献类型： 外文期刊

第一作者： Wang, Jinfang

作者： Wang, Jinfang;Wang, Yanping;Yu, Yongtao;Zhang, Jie;Ren, Yi;Tian, Shouwei;Li, Maoying;Liao, Shengjin;Guo, Shaogui;Gong, Guoyi;Zhang, Haiying;Xu, Yong

作者机构：

关键词： fruit ripening; SnRK2; 3; sucrose; watermelon

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Watermelon (Citrullus lanatus) as non-climacteric fruit is domesticated from the ancestors with inedible fruits. We previously revealed that the abscisic acid (ABA) signaling pathway gene ClSnRK2.3 might influence watermelon fruit ripening. However, the molecular mechanisms are unclear. Here, we found that the selective variation of ClSnRK2.3 resulted in lower promoter activity and gene expression level in cultivated watermelons than ancestors, which indicated ClSnRK2.3 might be a negative regulator in fruit ripening. Overexpression (OE) of ClSnRK2.3 significantly delayed watermelon fruit ripening and suppressed the accumulation of sucrose, ABA and gibberellin GA(4). Furthermore, we determined that the pyrophosphate-dependent phosphofructokinase (ClPFP1) in sugar metabolism pathway and GA biosynthesis enzyme GA20 oxidase (ClGA20ox) could be phosphorylated by ClSnRK2.3 and thereby resulting in accelerated protein degradation in OE lines and finally led to low levels of sucrose and GA(4). Besides that, ClSnRK2.3 phosphorylated homeodomain-leucine zipper protein (ClHAT1) and protected it from degradation to suppress the expression of the ABA biosynthesis gene 9'-cis-epoxycarotenoid dioxygenase 3 (ClNCED3). These results indicated that ClSnRK2.3 negatively regulated watermelon fruit ripening by manipulating the biosynthesis of sucrose, ABA and GA(4). Altogether, these findings revealed a novel regulatory mechanism in non-climacteric fruit development and ripening.

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