Pyruvate transporter BnaBASS2 impacts seed oil accumulation in Brassica napus

文献类型： 外文期刊

第一作者： Tang, Shan

作者： Tang, Shan;Guo, Ning;Tang, Qingqing;Peng, Fei;Liu, Yunhao;Xia, Hui;Lu, Shaoping;Guo, Liang;Tang, Shan;Guo, Ning;Tang, Qingqing;Peng, Fei;Liu, Yunhao;Xia, Hui;Lu, Shaoping;Guo, Liang;Guo, Liang;Guo, Liang

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关键词： Brassica napus; pyruvate transporter; BASS2; seed oil content; lipids; metabolites

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.263； 五年影响因子：11.619 ）

ISSN： 1467-7644

年卷期： 2022 年 20 卷 12 期

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收录情况： SCI

摘要： Bile acid: sodium symporter family protein 2 (BASS2) is a sodium-dependent pyruvate transporter, which transports pyruvate from cytosol into plastid in plants. In this study, we investigated the function of chloroplast envelope membrane-localized BnaBASS2 in seed metabolism and seed oil accumulation of Brassica napus (B. napus). Four BASS2 genes were identified in the genome of B. napus. BnaA05.BASS2 was overexpressed while BnaA05.BASS2 and BnaC04.BASS2-1 were mutated by CRISPR in B. napus. Metabolite analysis revealed that the manipulation of BnaBASS2 caused significant changes in glycolysis-, fatty acid synthesis-, and energy-related metabolites in the chloroplasts of 31 day-after-flowering (DAF) seeds. The analysis of fatty acids and lipids in developing seeds showed that BnaBASS2 could affect lipid metabolism and oil accumulation in developing seeds. Moreover, the overexpression (OE) of BnaA05.BASS2 could promote the expression level of multiple genes involved in the synthesis of oil and the formation of oil body during seed development. Disruption of BnaA05.BASS2 and BnaC04.BASS2-1 resulted in decreasing the seed oil content (SOC) by 2.8%-5.0%, while OE of BnaA05.BASS2 significantly promoted the SOC by 1.4%-3.4%. Together, our results suggest that BnaBASS2 is a potential target gene for breeding B. napus with high SOC.

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