Molecular Cloning, Tissue Distribution and Expression of Porcine y(+)L Amino Acid Transporter-1
文献类型: 外文期刊
第一作者: Zhi, Ai-min
作者: Zhi, Ai-min;Zuo, Jian-jun;Zou, Shi-geng;Huang, Zhi-yi;Wang, Xiao-lan;Tao, Lin;Feng, Ding-yuan;Zhi, Ai-min;Zhou, Xiang-yan
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关键词: tissue distribution
期刊名称:ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES ( 影响因子:2.509; 五年影响因子:2.604 )
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收录情况: SCI
摘要: In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human y+LAT I and by using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine y(+)LAT1 was isolated from porcine intestine RNA. It was 2,111 bp long, encoding a 511 amino acid trans-membrane glycoprotein composed of 12 transmembrane domains. The predicted amino acid sequence was found to be 91%, 90%, 87% and 87% identical to those of cattle, human, mouse and rat y(+)LAT1 respectively. Real-time RT-PCR results indicated that the small intestine had the highest y(+)LAT1 mRNA abundance and the lung had the lowest y(+)LAT1 mRNA abundance. Baby hamster kidney (BHK) cells transfected with green fluorescent protein (GFP) tagged porcine y(+)LAT1 cDNA indicated that the cellular localization of the gene product in BHK was on the plasma membrane.
分类号: Q95
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