Unique sequence characteristics of genes in the leftmost region of unique long region in duck enteritis virus

文献类型： 外文期刊

第一作者： Liu, Xiaoli

作者： Liu, Xiaoli;Liu, Shengwang;Li, Huixin;Han, Zongxi;Shao, Yuhao;Kong, Xiangang

作者机构：

关键词： Animals;Cluster Analysis;DNA;Viral;*DNA;Viral;Ducks;*Herpesviridae;Mardivirus;Molecular Sequence Data;Open Reading Frames;Phylogeny;Polymerase Chain Reaction;Sequence Analysis;DNA;Sequence Homology;Varicellovirus

期刊名称：INTERVIROLOGY （ 影响因子：1.763； 五年影响因子：1.929 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： OBJECTIVE: Our objective was to further understand the evolutionary position of duck enteritis virus (DEV) in the family Herpesviridae and to determine the genomic structure in the leftmost region of the unique long region (UL) of DEV. METHODS: 'Targeted gene walking PCR' was used to amplify the unknown gene of DEV clone-03 strain adjacent to known sequences in the UL region. Open reading frames (ORFs) were determined by Gene Runner and an online transcriptional element search engine. The phylogenetic tree was constructed by DNAStar based on the deduced amino acid sequences. RESULTS: A fragment of 13,947 bp, containing 5 ORFs (designated ICP4, ORF1, ORF2, LORF2 and LORF3, respectively), was amplified from the DEV clone-03 strain genome. In addition, a tandem repeat region of alpha-type-like sequences was detected between ICP4 and LORF2. Phylogenetic analysis of ICP4 indicated that DEV ICP4 should be clustered into Varicellovirus. DEV ORF1 and ORF2 were unique among herpesviruses. Additionally, the existence and characteristics of LORF2 and LORF3 indicated a close relationship with Mardivirus members. CONCLUSION: The characteristics and gene content in the leftmost end of the UL region of the DEV genome showed that the DEV genome is different from those of other herpesviruses.

分类号： Q93

  • 相关文献

[1]Apoptosis Induced by Duck Reovirus p10.8 Protein in Primary Duck Embryonated Fibroblast and Vero E6 Cells. Geng, Hongwei,Zhang, Yun,Guo, Dongchun,Wang, Yu,Liu, Ming,Zhang, Yun,Liu-Partanen, Yin,Tong, Guangzhi.

[2]Association between PCR-SSCP of bone morphogenetic protein 15 gene and prolificacy in Jining Grey goats. Chu, M. X.,Jiao, C. L.,He, Y. Q.,Wang, J. Y.,Liu, Z. H.,Chen, G. H..

[3]Haloactinobacterium album gen. nov., sp. nov., a halophilic actinobacterium, and proposal of Ruaniaceae fam. nov.. Tang, Shu-Kun,Zhi, Xiao-Yang,Wu, Jin-Yuan,Xu, Li-Hua,Li, Wen-Jun,Tang, Shu-Kun,Zhi, Xiao-Yang,Wu, Jin-Yuan,Xu, Li-Hua,Li, Wen-Jun,Wang, Yun,Lou, Kai,Lee, Jae-Chan,Kim, Chang-Jin.

[4]Disruption effect of Microplitis bicoloratus polydnavirus EGF-like protein, MbCRP, on actin cytoskeleton in lepidopteran insect hemocytes. Luo, Kai-Jun,Pang, Yi.

[5]Prolactin receptor as a candidate gene for prolificacy of small tail han sheep. Chu, M. X.,Mu, Y. L.,Fang, L.,Ye, S. C.,Sun, S. H..

[6]Molecular cloning, characterization and expression analysis of a clip-domain serine protease from pearl oyster Pinctada fucata. Xu, Xinping,Xu, Xinping,Zhang, Dianchang,Jiang, Shigui,Ma, Jianjun,Jiang, Jingjing,Pan, Dequan,Zhang, Dianchang,Jiang, Jingjing.

[7]Molecular identification, polymorphism, and expression analysis of major histocompatibility complex class IIA and B genes of turbot (Scophthalmus maximus). Zhang, Yu-Xi,Chen, Song-Lin.

[8]Use of mitochondrial RNA genes for the differentiation of four Trichinella species by multiplex PCR amplification. Blaga, R.,Fu, BaoQuan,Le Rhun, D.,Le Naour, E.,Heckman, A.,Zocevic, A.,Boireau, P.,Liu, MingYuan.

[9]The genetic analysis and germplasm identification of the gametophytes of Undaria pinnatifida (Phaeophyceae) with RAPD method. Wang, Di,Wang, Xiuliang,Li, Dapeng,Wang, Feijiu,Duan, Delin.

[10]White spot syndrome virus quantification in blue crab Portunus trituberculatus hatchery-produced larvae and wild populations by TaqMan real-time PCR, with an emphasis on the relationship between viral infection and crab health. Meng, Xian-Hong,Jang, In-Kwon,Seo, Hyung-Chul,Cho, Yeong-Roc,Meng, Xian-Hong.

[11]Overexpression of SIRT6 in Porcine Fetal Fibroblasts Attenuates Cytotoxicity and Premature Senescence Caused by D-Galactose and Tert-Butylhydroperoxide. Xie, Xiaoxian,Zhang, Hao,Wang, Liangliang,Zhang, Ailing,Li, Jiaqi,Xie, Xiaoxian,Zhang, Hao,Wang, Liangliang,Zhang, Ailing,Li, Jiaqi,Gao, Ping,Xie, Shuihua.

[12]Diazotrophic diversity, nifH gene expression and nitrogenase activity in a rice paddy field in Fujian, China. Martensson, Lotta,Diez, Beatriz,Wartiainen, Ingvild,El-Shehawy, Rehab,Rasmussen, Ulla,Zheng, Weiwen.

[13]Genetic differentiation in seven geographic populations of the fleshy shrimp Penaeus (Fenneropenaeus) chinensis based on microsatellite DNA. Meng, Xian Hong,Wang, Qing Yin,Liu, Ping,Kong, Jie,Meng, Xian Hong,Jang, In Kwon.

[14]Molecular cloning and characterization of Bombyx mori CREB gene. Song, Hongsheng,Sun, Yue,Zhang, Yang,Li, Muwang.

[15]Characterization of an intracellular cystatin homolog from the tick Haemaphysalis longicornis. Zhou, Jinlin,Liao, Min,Ueda, Mami,Fujisaki, Kozo,Zhou, Jinlin,Gong, Haiyan,Xuan, Xuenan,Fujisaki, Kozo.

[16]A novel phytase appA from Citrobacter amalonaticus CGMCC 1696: gene cloning and overexpression in Pichia pastoris. Luo, Huiying,Huang, Huoqing,Yang, Peilong,Wang, Yaru,Yuan, Tiezheng,Wu, Ningfeng,Yao, Bin,Fan, Yunliu.

[17]A new method for SNP discovery. Xu, Jian-Yong,Xu, Gen-Bo,Chen, Song-Lin,Xu, Jian-Yong,Xu, Gen-Bo.

[18]A Novel DNA Extraction Method for the Detection of Jujube Witches' Broom Phytoplasmas. Yu, X. M.,Zhou, G. F.,Shan, G. H.,Wang, H. R.,An, M.,Shen, G. N.,Lv, F. F.,Zhu, E. Y..

[19]Fine mapping of a quantitative trait locus qHD3-1, controlling the heading date, to a 29.5-kb DNA fragment in rice. Wang, W. Y.,Liu, X.,Ding, H. F.,Liu, W.,Yao, F. Y.,Jiang, M. S.,Li, G. X.,Zhu, C. X..

[20]Genetic effects and heterosis of the fibre colour and quality of brown cotton (Gossypium hirsutum). Hong-Jie FENG,Jun-Ling SUN,Jie WANG,Ying-Hua JIA,Xin-Yu ZHANG,Bao-Ying PANG,Jie SUN,Xiong-Ming DU.

作者其他论文 更多>>

微信小程序