Multiple amino acid substitutions are involved in the adaptation of H9N2 avian influenza virus to mice

文献类型： 外文期刊

第一作者： Wu, Rui

作者： Wu, Rui;Yang, Keli;Liang, Wangwang;Xiong, Zhongliang;Liu, Zewen;Yang, Xuehai;Shao, Huabin;Zheng, Xinmin;Chen, Mingxin;Xu, Diping;Wu, Rui;Xu, Diping;Wu, Rui;Zhang, Hongbo

作者机构：

关键词： nuclear localization;growth characteristic;virus adaptation;multiple amino acid substitution

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To explore adaptation of avian influenza virus to mice we previously performed serial lung-to-lung passages of the influenza A/Chicken/Jiangsu/7/2002 (H9N2) strain, resulting in the isolation of a variant influenza strain lethal for mice. We now report that virulence correlates with improved growth characteristics on mammalian cells and extended tissue tropism in vivo. Sequencing of the complete genomes of the wild-type and mouse-adapted viruses revealed 25 amino acid substitutions. Some were found to reiterate known substitutions in human and swine H9N2 influenza isolates. Functions affected include nuclear localization signals and sites of protein and RNA interaction, while others are known determinants of pathogenicity and host specificity such as the viral polymerase PB2 E627K substitution. These observations suggest that enhanced growth characteristics and modified cell tropism may contribute to increased virulence in mice. We conclude that multiple amino acid substitutions are likely to be involved in the adaptation of H9N2 avian influenza virus to mice.

分类号： S8

  • 相关文献

[1]A Novel Pepper (Capsicum annuum L.) WRKY Gene, CaWRKY30, Is Involved in Pathogen Stress Responses. Zheng Jingyuan,Mao Zhenchuan,Xie Bingyan,Zheng Jingyuan,Zou Xuexiao.

[2]Effects of the nuclear localization of the N-pro protein of classical swine fever virus on its virulence in pigs. Li, Yongfeng,Shen, Liang,Sun, Yuan,Wang, Xiao,Li, Chao,Huang, Junhua,Chen, Jianing,Li, Lianfeng,Zhao, Bibo,Luo, Yuzi,Li, Su,Qiu, Hua-Ji.

[3]CkDREB gene in Caragana korshinskii is involved in the regulation of stress response to multiple abiotic stresses as an AP2/EREBP transcription factor. Wang, Xuemin,Chen, Xiaofang,Gao, Hongwen,Wang, Zan,Sun, Guizhi,Liu, Yun.

[4]A new Akirin1 gene in turbot (Scophthalmus maximus): Molecular cloning, characterization and expression analysis in response to bacterial and viral immunological challenge. Yang, Chang-Geng,Wang, Xian-Li,Wang, Lei,Zhang, Bo,Chen, Song-Lin,Yang, Chang-Geng.

作者其他论文 更多>>

微信小程序