Protease-resistant core form of Bacillus thuringiensis Cry1Ie: monomeric and oligomeric forms in solution
文献类型: 外文期刊
第一作者: Zhang, Jie
作者: Zhang, Jie;Guo, Shuyuan;Zhang, Yancai;Huang, Dafang
作者机构:
关键词: Bacillus thuringiensis;Cry1Ie protein;Oligomer;Protease-resistant core form
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
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收录情况: SCI
摘要: Cry1Ie is encoded by cry1Ie, a gene that is silent in Bacillus thuringiensis strains but can be over-expressed in Escherichia coli. A protease-resistant core form of Cry1Ie with a molecular weight of approx. 55 kDa was found among the products of trypsin digestion. The oligomer and monomer of the protease-resistant core form were purified separately. The oligomer fraction contained a small quantity of dimer and a large amount of aggregates larger than tetramers. The 50% lethal concentrations (LC) of the full-length Cry1Ie and both the monomer and oligomer fractions of the protease-resistant core form against Plutella xylostella were 14, 21 and 1400 og/ml, respectively.
分类号: Q7
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