Phylogenetic relationship of two popular edible Pleurotus in China, Bailinggu (P-eryngii var. tuoliensis) and Xingbaogu (P-eryngii), determined by ITS, RPB2 and EF1 alpha sequences

文献类型： 外文期刊

第一作者： Li, Qian

作者： Li, Qian;Peng, Wei-Hong;Huang, Zhong-Qian;Gan, Bing-Cheng;Wu, Bo

作者机构：

关键词： Oyster mushroom; Awei mo; Ferula; Speciation

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2016 年 43 卷 6 期

页码：

收录情况： SCI

摘要： The aims of this study are to assess the utility of the internal transcribed spacer (ITS) region, and partial translation elongation factor (EF1 alpha) and RNA polymerase II (RPB2) genes, for differentiation of Bailinggu, P. eryngii, and P. nebrodensis; to reconstruct phylogenetic relationships between the three species; and to confirm the taxonomic status of Bailinggu based on ribosomal and protein-coding genes. Pairwise genetic distances between Bailinggu, P. eryngii, and related Pleurotus strains were calculated by using the p-distance model, and molecular phylogeny of these isolates was estimated based on ITS, RPB2, and EF1 alpha using maximum parsimony and Bayesian methods. Differences in ITS, RPB2, and EF1 alpha sequences show that Bailinggu, P. eryngii, and P. nebrodensis are distinct at the species level. Phylogenetic analyses reveal that P. eryngii is closer to P. nebrodensis than to Bailinggu. Sequence analyses of ribosomal and protein-coding genes confirm that P. eryngii var. tuoliensis is identical to Bailinggu. P. eryngii var. tuoliensis should be raised to species level or a new name should be introduced for Bailinggu after a thorough investigation into Pleurotus isolates from Ferula in Xinjiang Province. This study helps to resolve uncertainty regarding Bailinggu, P. eryngii and P. nebrodensis, improving the resource management of these strains. ITS, EF1 alpha, and RPB2 sequences can be used to distinguish Bailinggu, P. eryngii and P. nebrodensis as three different species, and P. eryngii var. tuoliensis should be the scientific name for Bailinggu at present.

分类号： Q7

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