UDP-glycosyltransferases-mediated vitellogenin protein biogenesis reveals juvenile hormone I-specific dominance in Spodoptera frugiperda reproductive programming

文献类型： 外文期刊

第一作者： Luo, Jie

作者： Luo, Jie;Ouyang, Huili;Wu, Haiyan;Yang, Taiyi;Li, Honghong;Li, Xuesheng;Xiong, Faqian;Yang, Taiyi

作者机构：

关键词： Juvenile hormone; Fall armyworm; Vitellogenin; UDP-glycosyltransferase

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 313 卷

页码：

收录情况： SCI

摘要： Juvenile hormone (JH) critically regulates reproduction in Spodoptera frugiperda (FAW) via its signaling cascade. We dissected isoform-specific roles of JH I, II, and III by using hormone injection, RNAi, and RNA-seq. Systematic modulation of JH variants revealed distinct roles in ovarian development, egg production, and vitellogenin (Vg) dynamics. Transcriptomic profiling identified JH I as the most potent inducer of reproductive pathways, specifically enriching the steroid hormone biosynthesis pathway. Within this pathway, two UDPglycosyltransferases (UGT), SfUGT2 and SfUGT2-like, were identified as key regulators of Vg synthesis through RNAi knockdown and functional validation (qPCR, Western blot). Silencing either gene reduced Vg levels and impaired ovarian maturation. JH I exhibited the strongest induction of SfUGT2/SfUGT2-like expression, correlating with its superior reproductive activation. Notably, JH III synergistically amplified JH I/II-induced Vg accumulation and egg production, as evidenced by co-treatment assays. This cooperative interplay highlights a tiered regulatory network among JH isoforms, and provide novel insights into the endocrine regulation of reproduction in FAW, also highlight the potential for targeted manipulation of JH signaling for pest control strategies.

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