A bacterial protein Rhp-PSP inhibits plant viral proliferation through endoribonuclease activity

文献类型： 外文期刊

第一作者： Chen, Lijie

作者： Chen, Lijie;Zhang, Deyong;Su, Pin;Liu, Yong;Chen, Lijie;Peng, Qianze;Du, Xiaohua;Zhang, Weixing;Cheng, Ju'e;Sun, Shu'e;Zhang, Deyong;Su, Pin;Liu, Yong

作者机构：

关键词： Rhodopseudomonas palustris; Rhp-PSP; tobacco mosaic virus; endoribonuclease; antiviral activity

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.6； 五年影响因子：4.5 ）

ISSN： 2095-3119

年卷期： 2024 年 23 卷 6 期

页码：

收录情况： SCI

摘要： Plant virus causes massive crop losses globally. However, there is currently no effective measure to control plant viral disease. Previously, we identify an antiviral protein Rhp-PSP, produced by the bacterial Rhodopseudomonas palustris strain JSC-3b. In this study, we discover that the antiviral activity of Rhp-PSP relies on its endoribonuclease activity. Converting the arginine (R) residue at position 129 onto alanine (A) abolishs its endoribonuclease activity on coat protein (CP) RNA of tobacco mosaic virus (TMV), consequentially, compromises the antiviral activity of RhpPSP. Further investigation demonstrates that, the mutant Rhp-PSP R129A is unable to form the homotrimer as the wild type, indicating the importance of quaternary junction for the endoribonuclease activity. Overexpression of RhpPSP in Nicotiana benthamiana significantly enhances the resistance against TMV of seedlings, while expression of Rhp-PSP R129A did not, confirming that endoribonuclease activity is responsible for the antiviral activity of Rhp-PSP. In addition, foliar spray of Rhp-PSP solution on tomato and pepper plants significantly reduces the disease index of viral diseases, indicating that Rhp-PSP shows potential to develop antiviral agent in practice.

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