Surface molecularly imprinted solid-phase extraction for the determination of vancomycin in plasma samples using HPLC-MS/MS

文献类型： 外文期刊

第一作者： Zhou, Hao

作者： Zhou, Hao;Chen, Qianqian;He, Limin;Liu, Rong;Zhou, Hao;Song, Xuqin

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关键词： LC-MS/MS; Plasma; Surface molecularly imprinted polymers; Solid-phase extraction; Vancomycin

期刊名称：ANALYTICAL SCIENCES （ 影响因子：1.967； 五年影响因子：1.675 ）

ISSN： 0910-6340

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收录情况： SCI

摘要： Vancomycin is a glycopeptide antibiotic used to treat infections caused by Gram-positive bacteria. Due to the narrow therapeutic index of vancomycin, it is necessary to develop a sensitive and reliable analytical method to monitor the drug concentration in plasma. A novel method based on surface molecularly imprinted solid-phase extraction combined with liquid chromatography-tandem mass spectrometry for the determination of vancomycin in plasma sample was developed. The plasma sample was cleaned up through the solid-phase extraction process before the analysis. The calibration standard of vancomycin in plasma ranged between 1 and 100 ng/mL, and the correlation coefficient (r) was 0.9993. The average recoveries were from 94.3 to 104.0%, and the precision was less than 10.5%. The limit of detection and limit of quantification were 0.5 ng/mL and 1 ng/mL, respectively. The method validated was successfully used for the detection of vancomycin in mice after oral administration.

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