Construction, Characterization, and Expressed Sequence Tag (EST) Analysis of Normalized cDNA Library of Thermo-Photoperiod-Sensitive Genic Male Sterile (TPGMS) Wheat from Spike Developmental Stages

文献类型： 外文期刊

第一作者： Yang, D.

作者： Yang, D.;Tang, Z. H.;Zheng, Y. L.;Tang, Z. H.;Zhang, L. P.;Zhao, C. P.

作者机构：

关键词： expressed sequence tags;wheat;androecium;plant fertility;photoperiod;complementary DNA;lines;heterosis;Triticum

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Thermo-photoperiod-sensitive genic male sterile (TPGMS) wheat is important in utilization of heterosis. To facilitate the use of such wheat line in agriculture, more knowledge about molecular mechanisms of TPGMS genes is required. In this study, we set up a normalized complementary DNA (cDNA) library based on the strategy of saturation hybridization with genomic DNA using TPGMS wheat line. This normalized cDNA library consists of cDNA from six directionally cloned cDNA libraries constructed with spike and anther tissues from spike developmental stages. From the normalized cDNA library, 3,264 single-pass expressed sequence tag (EST) were obtained. Exclusion of sequences shorter than 100 bp resulted in 3,223 vector-trimmed ESTs with a mean length of 926 bp. Clustering and assembly analysis resulted in 2,175 unique ESTs from 423 contigs and 1,752 singletons. Taking advantage of various tools and database, gene function classification showed that 60% of the ESTs were predicted to have putative gene function. Of the 2,175 unique ESTs, 264 (12%) displayed significant homology (BlastX E values <10e) to genes previously reported to be involved in cold-response related processes. Among these, sequences encoding activities related to primary metabolism, signal transduction, and transcriptional regulation were observed. Finally, in the total EST sequences, 108 potential SSRs were found. The unigene dataset will now be used to fabricate biochips carrying all identified genes for TPGMS wheat functional genomic research.

分类号： Q94

  • 相关文献

[1]Cytoplasmic male sterility with self-incompatibility, a novel approach to utilizing heterosis in rapeseed (Brassica napus L.). Wang, Han-zhong,Shen, Jin-xiong,Fu, Ting-dong,Tian, Bao-ming.

[2]Differential gene expression of cotton cultivar CCRI24 during somatic embryogenesis. Xiuming Wu,Fuguang Li#2;,Chaojun Zhang,Chuanliang Liu,Xueyan Zhang.

[3]Functional markers in wheat: current status and future prospects. Liu, Yanan,He, Zhonghu,Xia, Xianchun,He, Zhonghu,Appels, Rudi.

[4]Genetic diversity among a founder parent and widely grown wheat cultivars derived from the same origin based on morphological traits and microsatellite markers. Li, X. J.,Xu, X.,Yang, X. M.,Li, X. Q.,Liu, W. H.,Gao, A. N.,Li, L. H.,Li, X. J.,Xu, X..

[5]Heterosis Expression of Hybrid Rice in Natural-and Short-Day Length Conditions. Zhao Bing-ran,Lv Qi-ming,Fu Xi-qin,Xin Ye-yun,Yuan Long-ping. 2015

[6]Contribution of common wheat protein fractions to dough properties and quality of northern-style Chinese steamed bread. Zhang, Pingping,He, Zhonghu,Chen, Dongsheng,Zhang, Yong,Larroque, Oscar R.,Xia, Xianchun.

[7]Identification and characterization of reverse transcriptase domain of mranscriptionally active retrotransposons in wheat genomes. Tang, YM,Ma, YZ,Li, LC,Ye, XG. 2005

[8]Relationships between soil respiration and photosynthesis-related spectral vegetation indices in two cropland ecosystems. Huang, Ni,Niu, Zheng,Zhan, Yulin,Xu, Shiguang,Wu, Chaoyang,Gao, Shuai,Hou, Xuehui,Cai, Dewen,Huang, Ni,Xu, Shiguang,Hou, Xuehui,Cai, Dewen,Tappert, Michelle C.,Huang, Wenjiang.

[9]Effect of alternative tillage and residue cover on yield and water use efficiency in annual double cropping system in North China Plain. He Jin,Wang Qingjie,Li Hongwen,Gao Huanwen,Liu Lijin.

[10]Study on the interaction between 3 flavonoid compounds and alpha-amylase by fluorescence spectroscopy and enzymatic kinetics. Li, Y.,Gao, F.,Gao, F.,Zhao, C.,Shan, F.,Bian, J..

[11]Discovery, evaluation and distribution of haplotypes of the wheat Ppd-D1 gene. Guo, Zhiai,Song, Yanxia,Zhou, Ronghua,Jia, Jizeng,Guo, Zhiai,Ren, Zhenglong.

[12]Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique. Luo, M,Kong, XY,Jiang, T,Jia, C,Zhou, RH,Jia, JZ.

[13]Improvement of combining ability for restorer lines with the identified SSR markers in hybrid rice breeding. Liu, XC,Chen, SG,Chen, JS,Ishiki, KS,Wang, WX,Yu, LQ.

[14]Application of identified QTL-marker associations in rice quality improvement through a design-breeding approach. Wang, Jiankang,Wan, Xiangyuan,Li, Huihui,Pfeiffer, Wolfgang H.,Crouch, Jonathan,Wan, Jianmin.

[15]The integration of a macrophage-adapted live vaccine strain of equine infectious anaemia virus (EIAV) in the horse genome. Liu, Qiang,Wang, Xue-Feng,Du, Cheng,Lin, Yue-Zhi,Ma, Jian,Zhou, Jian-Hua,Wang, Xiaojun,Wang, Yu-Hong. 2017

[16]PROTOPLASMIC FACTORS, ANTIOXIDANT RESPONSES, AND CHILLING RESISTANCE IN MAIZE. ZHANG, JX,CUI, SP,LI, JM,WEI, JK,KIRKHAM, MB.

[17]Development of Chromosome Segment Substitution Lines Derived from Backcross between Two Sequenced Rice Cultivars, Indica Recipient 93-11 and Japonica Donor Nipponbare. Zhu, Wenyin,Lin, Jing,Yang, Dewei,Zhao, Ling,Zhang, Yadong,Zhu, Zhen,Chen, Tao,Wang, Cailin.

[18]Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza sativa L.. Qin, Qiu-lin,Liu, Jin-ge,Zhang, Zhen,Peng, Ri-he,Xiong, Ai-sheng,Yao, Quan-hong,Chen, Jian-min.

[19]Xuhuai Goat H-FABP Gene Clone, Subcellular Localization of Expression Products and the Preparation of Transgenic Mice. Yin, Yan-hui,Li, Bi-chun,Wei, Guang-hui,Zhu, Cai-ye,Li, Wei,Zhang, Ya-ni,Du, Li-xin,Cao, Wen-guang.

[20]Molecular Characterization and Expression Pattern of Two General Odorant Binding Proteins from the Diamondback Moth, Plutella xylostella. Zhang, Zhi-Chun,Wang, Man-Qun,Zhang, Guoan,Lu, Yao-Bin.

作者其他论文 更多>>

微信小程序