Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris

文献类型： 外文期刊

第一作者： Teng, Da

作者： Teng, Da;Fan, Ying;Yang, Ya-lin;Tian, Zi-gang;Luo, Jin;Wang, Jian-hua

作者机构：

关键词： beta-1;3-1;4-glucanase;codon optimization;Bacillus licheniformis;Expression;Pichia pastoris (P. pastoris);BETA-GLUCANASE GENE;METHYLOTROPHIC YEAST;ENDO-BETA-1;3-1;4-GLUCANASE GENE;HETEROLOGOUS PROTEINS;ESCHERICHIA-COLI;SECRETION;SUBTILIS;XYLANASE;BARLEY;CONSTRUCTION

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

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年卷期：

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收录情况： SCI

摘要： beta-1,3-1,4-glucanase (EC3.2.1.73) as an important industrial enzyme has been widely used in the brewing and animal feed additive industry. To improve expression efficiency of recombinant beta-1,3-1,4-glucanase from Bacillus licheniformis EGW039(CGMCC 0635) in methylotrophic yeast Pichia pastoris GS115, the DNA sequence encoding beta-1,3-1,4-glucanase was designed and synthesized based on the codon bias of P. pastoris, the codons encoding 96 amino acids were optimized, in which a total of 102 nucleotides were changed, the G+C ratio was simultaneously increased from 43.6 to 45.5%. At shaking flask level, beta-1,3-1,4-glucanase activity is 67.9 and 52.3 U ml(-1) with barley beta-glucan and lichenan as substrate, respectively. At laboratory fermentor level, the secreted protein concentration is approximately 250 mg l(-1). The beta-1,3-1,4-glucanase activity is 333.7 and 256.7 U ml(-1) with barley beta-glucan and lichenan as substrate, respectively; however, no activity of this enzyme on cellulose is observed. Compared to the nonoptimized control, expression level of the optimized beta-1,3-1,4-glucanase based on preferred codons in P. pastoris shown a 10-fold higher level. The codon-optimized enzyme was approximately 53.8% of the total secreted protein. The optimal acidity and temperature of this recombinant enzyme were pH 6.0 and 45 degrees C, respectively.

分类号： Q93

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