Isolation and functional characterization of HvDREB1-a gene encoding a dehydration-responsive element binding protein in Hordeum vulgare

文献类型: 外文期刊

第一作者: Xu, Zhao-Shi

作者: Xu, Zhao-Shi;Ni, Zhi-Yong;Li, Zhi-Yong;Li, Lian-Cheng;Chen, Ming;Gao, Dong-Yao;Yu, Xiu-Dao;Liu, Pei;Ma, You-Zhi

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关键词: Abiotic stress;Barley;DREB;Induction kinetics;Salt tolerance;Transgenic plants;ARABIDOPSIS TRANSCRIPTION FACTOR;GOSSYPIUM-HIRSUTUM;STRESS TOLERANCE;ABSCISIC-ACID;FACTOR DREB2A;HIGH-SALT;EXPRESSION;DROUGHT;COLD;BARLEY

期刊名称:JOURNAL OF PLANT RESEARCH ( 影响因子:2.629; 五年影响因子:2.926 )

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收录情况: SCI

摘要: A gene encoding Hordeum vulgare dehydration-responsive element binding protein 1 (HvDREB1), a member of the A-2 subgroup of the DREB subfamily, was isolated from barley seedlings. A subcellular localization assay revealed accumulation of HvDREB1 protein in the nucleus. As a trans-acting factor, HvDREB1 was able to bind to DRE/CRT elements and transactivate reporter gene expression in yeast cells. A study of various deletion mutants of HvDREB1 proteins indicated that the transactivation activity was localized to the N-terminal region. Expression of the HvDREB1 gene in barley leaves was significantly induced by salt, drought, and low-temperature. In contrast to most A-2 subgroup members in Arabidopsis thaliana, HvDREB1 also responded to exogenous ABA. Overexpression of HvDREB1 activated a downstream gene, RD29A, under normal growth conditions and led to increased tolerance to salt stress in Arabidopsis plants. These results suggest that HvDREB1 produces a DRE-/CRT-binding transcription factor that may have an important role in improving salt tolerance in plants.

分类号: Q94

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