Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton

文献类型： 外文期刊

第一作者： Shang, Hai-Hong

作者： Shang, Hai-Hong;Liu, Chuan-Liang;Zhang, Chao-Jun;Li, Feng-Lian;Hong, Wei-Dong;Li, Fu-Guang

作者机构：

关键词： Callus;Cotton;Transformation;Morphogenesis;Callus;Cotton;Transformation;Morphogenesis;Genetic engineering;Ultrastructure;Organelles;Genetically engineered organisms;Starch grains;Plant breeding;Chloroplasts;Plastids;Cytoplasm;Nuclei;Extracellular matrix;Agrobacterium

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Over the past few decades genetic engineering has been applied to improve cotton breeding. Agrobacterium medicated transformation is nowadays widely used as an efficient approach to introduce exogenous genes into cotton for genetically modified organisms. However, it still needs to be improved for better transformation efficiency and higher embryogenic callus induction ratios. To research further the difference of mechanisms for morphogenesis between embryogenic callus and non-embryogenic callus, we carried out a systematical study on the histological and cellular ultrastructure of Agrobacterium transformed calli. Results showed that the embryogenic callus developed nodule-like structures, which were formed by small, tightly packed, hemispherical cells. The surface of some embryogenic callus was covered with a fibrilar-like structure named extracellular matrix. The cells of embryogenic calli had similar morphological characteristics. Organelles of embryogenic callus cells were located near the nucleus, and chloroplasts degraded to proplastid-like structures with some starch grains. In contrast, the non-embryogenic calli were covered by oval or sphere cells or small clusters of cells. It was observed that cells had vacuolation of cytoplasm and plastids with a well organized endomembrane system. This study aims to understand the mechanisms of embryogenic callus morphogenesis and to improve the efficiency of cotton transformation in future.

分类号： Q94

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