Chryseobacterium herbae Isolated from the Rhizospheric Soil of Pyrola calliantha H. Andres in Segrila Mountain on the Tibetan Plateau

文献类型： 外文期刊

第一作者： Zhang, Li

作者： Zhang, Li;Li, Yan;Zhang, Li;Wang, Yan;Kong, Delong;Ma, Qingyun;Ruan, Zhiyong;Wang, Yan;Xing, Zhen;Ma, Qingyun

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关键词： Chryseobacterium herbae sp. nov.; alpine ecosystems; rhizosphere soil; Gram-staining-negative; Pyrola calliantha H. Andres; genomic; shotgun proteomics

期刊名称：MICROORGANISMS （ 影响因子：4.5； 五年影响因子：4.8 ）

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年卷期： 2023 年 11 卷 8 期

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收录情况： SCI

摘要： A non-motile, Gram-staining-negative, orange-pigmented bacterium called herbae pc1-10(T) was discovered in Tibet in the soil around Pyrola calliantha H. Andres' roots. The isolate thrived in the temperature range of 10-30 degrees C (optimal, 25 degrees C), pH range of 5.0-9.0 (optimum, pH = 6.0), and the NaCl concentration range of 0-1.8% (optimal, 0%). The DNA G+C content of the novel strain was 37.94 mol%. It showed the function of dissolving organophosphorus, acquiring iron from the environment by siderophore and producing indole acetic acid. Moreover, the genome of strain herbae pc1-10(T) harbors two antibiotic resistance genes (IND-4 and AdeF) encoding a beta-lactamase, and the membrane fusion protein of the multidrug efflux complex AdeFGH; antibiotic-resistance-related proteins were detected using the Shotgun proteomics technology. The OrthoANIu values between strains Chryseobacterium herbae pc1-10(T); Chryseobacterium oleae CT348T; Chryseobacterium kwangjuense KJ1R5(T); and Chryseobacterium vrystaatense R-23566(T) were 90.94%, 82.96%, and 85.19%, respectively. The in silico DDH values between strains herbae pc1-10(T); C. oleae CT348(T); C. kwangjuense KJ1R5(T); and C. vrystaatense R-23566(T) were 41.7%, 26.6%, and 29.7%, respectively. Chryseobacterium oleae, Chryseobacterium vrystaatense, and Chryseobacterium kwangjuense, which had 16S rRNA gene sequence similarity scores of 97.80%, 97.52%, and 96.75%, respectively, were its closest phylogenetic relatives. Chryseobacterium herbae sp. nov. is proposed as the designation for the strain herbae pc1-10(T) (=GDMCC 1.3255 = JCM 35711), which represented a type species based on genotypic and morphological characteristics. This study provides deep knowledge of a Chryseobacterium herbae characteristic description and urges the need for further genomic studies on microorganisms living in alpine ecosystems, especially around medicinal plants.

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