Determination and Chemotaxonomic Analysis of Lanostane Triterpenoids in the Mycelia of Ganoderma spp. Using Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry (I)

文献类型： 外文期刊

第一作者： Yue, Yawen

作者： Yue, Yawen;Zhou, Shuai;Zhang, Jingsong;Feng, Na;Yue, Yawen;Han, Wei;Cheng, Chilu;Teng, Liming;Cui, Baokai;Dai, Yucheng

作者机构：

关键词： Ganodermataceae; Polyporales; ganoderic acids; static fermentation; quantitative analysis; chemotaxonomy

期刊名称：PLANTA MEDICA （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 0032-0943

年卷期： 2023 年

页码：

收录情况： SCI

摘要： A comprehensive and sensitive method combining ultra-performance liquid chromatography with tandem mass spectrometry was developed for the quantification of characteristic triterpenoids in Ganoderma mycelia. Eight ganoderic acids previously isolated from the mycelia of Ganoderma lingzhi were separated with a binary mobile phase on a reversed-phase C18 column. A triple quadrupole mass spectrometer equipped with an electrospray ionization source was used as the detector in the negative ion mode. Identification and quantitation of target ganoderic acids were accomplished using the dynamic multiple reaction monitoring mode. The developed method was validated in terms of linearity, precision, accuracy, stability, and recovery. The method was first applied to quantify the contents of eight ganoderic acids in the mycelia of G. lingzhi at different times to determine the optimum fermentation conditions. Subsequently, the distribution of triterpenoids and the contents of eight ganoderic acids in sixteen different Ganoderma species were investigated. The results indicated that UV chromatography combined with dynamic multiple reaction monitoring quantification was an effective chemotaxonomy method for Ganoderma species identification. This study also provided a helpful analytical methodology for both scientific and industrial applications in the quality control of Ganoderma triterpenoids.

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