Flexible Hydrophobic Paper-Based Microfluidic Field-Effect Biosensor Amplified by RNA-Cleaving DNAzyme-Based DNA Nanostructure for Mg2+ Detection

文献类型： 外文期刊

第一作者： Wang, Hui

作者： Wang, Hui;He, Yue;Yu, Zhixue;Chen, Ruipeng;Zhang, Fan;Nan, Xuemei;Mijit, Mukaddas;Xiong, Benhai;Yang, Liang;Tang, Xiangfang;Feng, Zemeng;Chen, Dongfei;Shakweer, Waleid Mohamed El-Sayed

作者机构：

关键词： hydrophobic paper; similar DNA origami; microfluidic chip; electrochemical electrode; DNA nanoparticle; magnesium ion

期刊名称：BIOSENSORS-BASEL （ 影响因子：5.6； 五年影响因子：5.7 ）

ISSN：

年卷期： 2025 年 15 卷 7 期

页码：

收录情况： SCI

摘要： Magnesium ions (Mg2+) play an important role in animal health, with their concentration in the bloodstream serving as a key indicator for hypomagnesemia diagnosis. In this study, a flexible hydrophobic paper-based microfluidic field-effect biosensor was developed for point-of-care Mg2+ detection, which integrated flexible hydrophobic paper, semiconducting single-walled carbon nanotubes (SWNTs) and a Mg2+-specific RNA-cleaving DNAzyme(RCD)-based DNA nanostructure. Flexible hydrophobic paper was synthesized by using cellulose paper and octadecyltrichlorosilane, improving mechanical strength and decreasing biological interference. To achieve high sensitivity, the Mg2+-specific RCD was functionalized with SWNTs, and then repeatedly self-assembled two different Y-shaped DNAs to construct a DNA nanostructure based on a similar DNA origami technique. This proposed biosensor exhibited a linear detection range from 1 mu M to 1000 mu M, with a detection limit of 0.57 mu M, demonstrating its great stability, selectivity, and anti-interference performance. This innovative design offers promising potential for Mg2+ monitoring in real applications.

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