Salmonid alphavirus non-structural protein 2 is a key protein that activates the NF-KB signaling pathway to mediate inflammatory responses

文献类型： 外文期刊

第一作者： Gao, Shuai

作者： Gao, Shuai;Liu, Xuefei;Han, Bing;Wang, Na;Guan, Xueting;Huang, Jinshan;Shi, Wen;Liu, Min;Lv, Xiaonan;Xu, Gefeng;Shi, Wen;Liu, Min

作者机构： Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China;Beijing Aquaculture Technol Extent Stn, Beijing 100176, Peoples R China;Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Key Lab Freshwater Aquat Biotechnol & Breeding, Minist Agr & Rural Affairs, Harbin 150070, Peoples R China;Northeast Agr Univ, Coll Anim Sci & Technol, Chang Jiang Rd 600, Harbin, Peoples R China

关键词： Salmonid alphavirus; NF-KB; TLR; Nsp2; Inflammation

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 2021影响因子：4.622； 五年影响因子：4.799 ）

ISSN： 1050-4648

年卷期： 2022 年 129 卷

页码：

收录情况： SCI

摘要： Salmonid alphavirus (SAV) infection of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) causes pancreas disease (PD) with typical inflammatory responses, such as necrosis of the exocrine pancreas, cardiomyopathy and skeletal myopathy. However, the pathogenic mechanism underlying SAV infection is still unclear. Inflammation may cause damage to the body, but it is a defense response against infection by pathogenic microorganisms, of which nuclear factor-kappa B (NF-KB) is the main regulator. This study revealed that SAV can activate NF-KB, of which the viral nonstructural protein Nsp2 is the major activating protein. SAV activates the NF-KB signaling pathway by simultaneously up-regulating TLR3, 7, 8 and then the expression of the signaling molecule myeloid differentiation factor 88 (Myd88) and tumor necrosis factor receptor-associated factor 6 (TRAF6). We found that Nsp2 can induce IKB degradation and p65 phosphorylation and transnucleation, and activate NF-KB downstream inflammatory cytokines. Nsp2 may simultaneously activate NF-KB through TLR3,7,8-dependent signaling pathways. Overexpression of Nsp2 can up-regulate mitochondrial antiviral signaling protein (MAVS) and then promote the expression of IFNa1 and antiviral protein Mx, which inhibits viral replication. This study shows that Nsp2 acts as a key activator protein for the NF-KB signaling pathway, which induces inflam-mation post-SAV infection. This study systematically analyzes the molecular mechanism of SAV activation of the NF-KB signaling pathway, and provides a theoretical basis for revealing the mechanism of innate immune response and inflammatory injury caused by SAV.

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