Gastrointestinal digestion fates of lipids based on pH-stat and Caco-2 cells models: Superior bioavailability of triglyceride monomer derived from silkworm pupae oil

文献类型： 外文期刊

第一作者： Yan, Cheng-Hai

作者： Yan, Cheng-Hai;Xun, Xiao-Meng;Herman, Richard A.;Zhan, Yu-Fan;Gong, Lu-Chan;Wu, Fu-An;Wang, Jun;Herman, Richard A.;Gong, Lu-Chan;Wu, Fu-An;Wang, Jun

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关键词： Silkworm pupae oil; Separation; Triacylglyceride monomer; Bioavailability; pH-stat; Caco-2 cells; In vitro

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.2； 五年影响因子：5.4 ）

ISSN： 2212-4292

年卷期： 2023 年 53 卷

页码：

收录情况： SCI

摘要： Silkworm pupae oil (SPO) was regarded as a good alternative to alpha-linolenic acid supplements, which contain a variety of triacylglyceride with high nutritional value. To investigate the connections between triacylglyceride composition and lipid gastrointestinal digestive absorption profiles, isolation and bioavailability of tri-acylglyceride monomers from silkworm pupae oil was analyzed. In this study, two triacylglyceride monomers rich in alpha-linolenic acid were separated by preparative liquid chromatography, namely LnLnLn (1,2,3-alpha-linolenic acid triacylglyceride) and LnLnL (1,2-alpha-linolenic acid, 3-linoleic acid triacylglyceride). The in vitro digestion in the pH-stat model results showed the digestive rate of LnLnLn was 0.4101 +/- 0.0089 s-1, which was significantly higher than that of SPO (0.2494 +/- 0.0108 s-1) (p < 0.05). The in vitro absorption in the Caco-2 cells model results showed the absorptivity of LnLnLn and LnLnL were 6.40 +/- 0.19% and 6.06 +/- 0.54%, which were significantly higher than SPO (5.43 +/- 0.51%) (p < 0.05). In addition, the mRNA expression levels of lipid metabolism-related proteins provide evidence for the absorption superiority of LnLnLn and LnLnL. The present work provides novel structural lipids for rapid and efficient supplementation of alpha-linolenic acid.

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