Study on interfacial regulation in real food system: Cellulase-induced steady-state reconstruction of flaxseed-based plant milk and its molecular mechanisms

文献类型： 外文期刊

第一作者： Hao, Qian

作者： Hao, Qian;Deng, Ziyu;Li, Ruyi;Cheng, Yuanmeng;Chen, Yashu;Deng, Qianchun;Pei, Yaqiong;Hao, Qian;Zhou, Bin

作者机构：

关键词： Flaxseed; Plant-based milk; Cellulase; Stability; Oil bodies

期刊名称：FOOD HYDROCOLLOIDS （ 影响因子：12.4； 五年影响因子：13.3 ）

ISSN： 0268-005X

年卷期： 2025 年 158 卷

页码：

收录情况： SCI

摘要： Cellulase is commonly used for the better release of phytonutrients due to its destructive coordinate effect on plant cell walls. In this study, cellulase hydrolysis showed a new feature: it significantly increased the physicochemical stability of flaxseed-based plant milk (FPM) due to interfacial modification of the oil bodies. Multispectroscopy and rheological measurement were performed to evaluate the stability of FPM, while the mechanical and oxidation stability of flaxseed oil bodies (FOBs) were characterized by rheological and induction period analysis, respectively. Additionally, Cryo-SEM, FTIR, and XRD analysis were used to provide structural information of FOBs' interface. Results revealed that cellulase treatment reduced the particle size and TSI of FPM, and significantly increased the adsorption of total polysaccharides at the FOBs' interface, with an increase of 20.25 +/- 3.77% (P < 0.05). Furthermore, FOBs with enzyme treatment (EN-FOBs) possessed higher elastic modulus (G ') and viscous modulus (G '') at 90 degrees C than untreated ones. Meanwhile, the induction period of the ENFOBs was prolonged by 10.47 +/- 2.10% (P < 0.05). These findings indicated that cellulase promoted steady-state reconstruction of FPM by contributing to formation of complex interfaces with high mechanical strength. These results offer insight into innovation in plant-based milk processing technology towards green, clean labeling, and high-quality.

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