Knockout of miR396 genes increases seed size and yield in soybean∞

文献类型： 外文期刊

第一作者： Xie, Hongtao

作者： Xie, Hongtao;Niu, Qingfeng;Geng, Leping;Deng, Yuanwei;Zhu, Jianhua;Xie, Hongtao;Niu, Qingfeng;Geng, Leping;Deng, Yuanwei;Zhu, Jianhua;Xie, Hongtao;Ji, Zhanbo;Pang, Kang;Su, Fei;Dong, Jinsong;Guo, Rui;Zhang, Yang;Cao, Xuesong;Song, Minglei;Zhu, Jian-Kang;Zheng, Zai

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关键词： Cas12SF01; CRISPR/Cas; miR396; seed size; soybean; yield

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Yield improvement has long been an important task for soybean breeding in the world in order to meet the increasing demand for food and animal feed. miR396 genes have been shown to negatively regulate grain size in rice, but whether miR396 family members may function in a similar manner in soybean is unknown. Here, we generated eight soybean mutants harboring different combinations of homozygous mutations in the six soybean miR396 genes through genome editing with clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated nuclease (Cas)12SF01 in the elite soybean cultivar Zhonghuang 302 (ZH302). Four triple mutants (mir396aci, mir396acd, mir396adf, and mir396cdf), two quadruple mutants (mir396abcd and mir396acfi), and two quintuple mutants (mir396abcdf and mir396bcdfi) were characterized. We found that plants of all the mir396 mutants produced larger seeds compared to ZH302 plants. Field tests showed that mir396adf and mir396cdf plants have significantly increased yield in growth zones with relatively high latitude which are suited for ZH302 and moderately increased yield in lower latitude. In contrast, mir396abcdf and mir396bcdfi plants have increased plant height and decreased yield in growth zones with relatively high latitude due to lodging issues, but they are suited for low latitude growth zones with increased yield without lodging problems. Taken together, our study demonstrated that loss-of-function of miR396 genes leads to significantly enlarged seed size and increased yield in soybean, providing valuable germplasms for breeding high-yield soybean.

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