Direct conversion of carbon dioxide to glucose using metabolically engineered Cupriavidus necator

文献类型： 外文期刊

第一作者： Wang, Xiaolu

作者： Wang, Xiaolu;Luo, Huiying;Wang, Yaru;Wang, Yuan;Tu, Tao;Qin, Xing;Su, Xiaoyun;Huang, Huoqing;Bai, Yingguo;Yao, Bin;Zhang, Jie;Zhang, Jie

作者机构：

关键词： Cupriavidus necator; Metabolic engineering; CO2; Glucose; HAD phosphatase

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.889； 五年影响因子：11.139 ）

ISSN： 0960-8524

年卷期： 2022 年 362 卷

页码：

收录情况： SCI

摘要： Artificial synthesis of glucose, the monomer of starch, from renewable resources and CO2 is a promising method for addressing food crisis and alleviating climate change. Here, the construction of a microbial biocatalyst for glucose production from renewable resources and CO2 was reported. Initially, blocking the glucose catabolic pathway via deletion of glk gene generated a glucose-producing strain of Cupriavidus necator with titers of 24.7, 47.5 and 180.1 mg/L from fructose, glycerol and CO2, respectively. Subsequently, the Entner-Doudoroff pathway and polyhydroxybutyrate biosynthesis pathway were disrupted to further increase glucose accumulation. The maximum glucose titer and yield on biomass from CO2 reached 253.3 mg/L and 91.6 mg/L/OD600, respectively. Finally, the phosphatases that mediate the dephosphorylation of phosphorylated glucose were identified. Overexpression of HAD1 and cbbY2 could enhance glucose titer by 5.5-fold when fructose was used as sole carbon source. This study demonstrates a feasible route for microbial-based synthesis of glucose from CO2.

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