Detection and integrated analysis of lncRNA and mRNA relevant to plateau adaptation of Yak

文献类型： 外文期刊

第一作者： Wang, Jiabo

作者： Wang, Jiabo;Chai, Zhixin;Deng, Lei;Wang, Jikun;Wang, Hui;Zhong, Jincheng;Wang, Jiabo;Chai, Zhixin;Wang, Jikun;Wang, Hui;Zhong, Jincheng;Tang, You;Ji, Qiumei

作者机构：

关键词： co-expression network; lncRNA; mRNA; plateau adaptation; Yak

期刊名称：REPRODUCTION IN DOMESTIC ANIMALS （ 影响因子：1.858； 五年影响因子：1.96 ）

ISSN： 0936-6768

年卷期： 2020 年 55 卷 11 期

页码：

收录情况： SCI

摘要： Known as the 'ship of the plateau', through thousands of years evolution and cruelty environments selection containing low oxygen and strong ultraviolet radiation, yaks have adapted plateau environments and supplied important goods and materials for the people in the Qinghai-Tibet Plateau. This study aimed to identify differentially expressed (DE) genes and novel long non-coding RNAs (lncRNAs) of yaks for the Plateau adaptation and their underlying co-expression and regulatory network. We carried out RNA-seq analysis for cerebral and cerebellar tissue specimens ofBos taurus,Bos grunniens x Bos TaurusandB. grunniens. Furthermore, 12,072 pseudo lncRNAs were predicted using three software. In total, 4,257 significant DE transcripts were identified using the Ballgown R package (p < .01), of which 1,021 were protein-coding genes, 14 were known lncRNAs, and 661 were novel lncRNAs. Using WGCNA, a co-expression network of DE mRNAs and lncRNAs comprising 5 modules was generated to determine functional associations clusters. This study reveals a valuable sub-network comprising 8 hub genes, one known lncRNA and 5 novel lncRNAs in the major module. These hub genes are associated with blood pressure regulation, generation of reactive oxygen species and metabolism. The analysis of co-expressed genes thus provides a basis for the regulatory mechanisms in PA in Yaks and for the detection of additional genes between cross-breed and parent populations.

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