Transgenic Wheat Plants Derived from Agrobacterium-mediated Transformation of Mature Embryo Tissues

文献类型： 外文期刊

第一作者： Wang, Y. L.

作者： Wang, Y. L.;Yin, G. X.;Tao, L. L.;Ye, X. G.;Xu, M. X.;Wang, D. W.

作者机构：

关键词： Agronomy: Agriculture

期刊名称：CEREAL RESEARCH COMMUNICATIONS （ 影响因子：0.85； 五年影响因子：1.067 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Although significant progress has been made on Agrobacterium-mediated wheat transformation, the current methodologies use immature embryos as recipient tissues, a process which is labor intensive, time consuming and expensive. In this study, we have managed to develop an Agrobacterium-based transformation scheme using explants derived from mature embryos. Based on transient expression of beta-glucuronidase (GUS) marker, mature embryo halves prepared from freshly imbibed seeds were generally most susceptible to Agrobacterium-mediated T-DNA transfer. According to the results of callus induction and shoot production, Yumai 66 and Lunxuan 208 showed higher selection and regeneration efficiency than Bobwhite. In line with this finding, fertile T-0 transgenic plants were most readily obtained for both spring and winter wheat when mature embryo halves were used for co-inoculation by Agrobacterium cells. The presence of the antibiotic selection marker (nptII, encoding neomycin phosphotransferase II) in the T-0 plants was revealed by both genomic PCR amplification and the enzyme-linked immunosorbent assay (ELISA). Additional analysis showed that the transgene was stably inherited from the two different generations and segregated normally among the T-1 progenies. Further development along this line will raise the efficiency of wheat transformation and increase the use of this approach in the molecular breeding of wheat crop.

分类号： S5

  • 相关文献

[1]Analysis of Quantitative Trait Loci for Panicle Layer Uniformity in Rice (Oryza sativa L.). Ma, L. Y.,Xiao, P.,Xia, Y. W.,Bao, J. S.,Ma, L. Y.,Xiao, P.,Xia, Y. W.,Bao, J. S.,Ma, L. Y.,Cai, J.,Li, X. M.,Ji, Z. J.,Yang, C. D..

[2]Molecular mapping of powdery mildew resistance gene PmHNK in winter wheat (Triticum aestivum L.) cultivar Zhoumai 22. Xu, W. G.,Li, C. X.,Hu, L.,Zhang, L.,Zhang, J. Z.,Dong, H. B.,Wang, G. S.,Li, C. X..

[3]Molecular Cloning and Expression of cDNA Encoding the Cysteine Proteinase Inhibitor from Upland Cotton. Jiang, Ming-feng,Li, Sheng-wei,Chen, Min,Li, Sheng-wei,Chen, Min,Cai, Ying-fan,Xie, Yong-fang,Li, Biao,Sun, Quan,Jiang, Huai-zhong,Pan, Zheng,Gao, Yun-ling,Yuan, You-Lu,Shi, Yu-zheng.

[4]Field-Testing of Synthetic Herbivore-Induced Plant Volatiles as Attractants for Beneficial Insects. Zhang, Yongjun,Wu, Kongming,Guo, Yu Yuan,Yu, Huilin.

[5]Characterization of Five Putative Acyl Carrier Protein (ACP) Isoforms from Developing Seeds of Arachis hypogaea L.. Li, Meng-Jun,Wang, Xing-Jun,Su, Lei,Bi, Yu-Ping,Wan, Shu-Bo.

[6]Development of SNP markers and haplotype analysis of the candidate gene for rhg1, which confers resistance to soybean cyst nematode in soybean. Li, Ying-Hui,Zhang, Chen,Ma, Zulu,Liu, Zhang-Xiong,Nan, Hai-Yang,Chang, Ru-Zhen,Qiu, Li-Juan,Gao, Zhong-Shan,Smulders, Marinus Johannes Maria,Ma, Zulu.

[7]The association of Sclerotinia sclerotiorum resistance with glucosinolates in Brassica napus double-low DH population. Fan, Z. X.,Sun, X. L.,Yu, B.,Wang, Y. Z.,Yang, G. S.,Lei, W. X.,Fan, Z. X..

[8]OsNOA1/RIF1 is a functional homolog of AtNOA1/RIF1: implication for a highly conserved plant cGTPase essential for chloroplast function. Liu, Hongjia,Li, Sujuan,Huang, Guo,Guo, Peng,Tao, Yuezhi,Liu, Hongjia,Chu, Hung,Lo, Clive,Lau, Edward,Lam, Maggie P. Y.,Chu, Ivan K.,Wang, Junqi,Jiang, Liwen.

作者其他论文 更多>>

微信小程序