Assessment of the cell-mediated immunity induced by alphavirus replicon-vectored DNA vaccines against classical swine fever in a mouse model

文献类型: 外文期刊

第一作者: Zhao, He-Ping

作者: Zhao, He-Ping;Sun, Jian-Fu;Li, Na;Sun, Yuan;Xia, Zhao-He;Wang, Yu;Cheng, Dan;Qi, Qiao-Fen;Jin, Mei-Lin;Qiu, Hua-Ji;Sun, Jian-Fu;Jin, Mei-Lin

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关键词: lymphoproliferative response;immunogenicity;cell-mediated immunity

期刊名称:VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY ( 影响因子:2.046; 五年影响因子:2.217 )

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收录情况: SCI

摘要: We have previously shown that an alphavirus replicon-vectored DNA vaccine (pSFV1CS-E2) encoding the E2 glycoprotein of classical swine fever virus (CSFV) completely protected the immunized pigs from lethal challenge. These animals developed only low or moderate level viral-specific antibody titers before challenge, implying that cell-mediated immunity (CMI) probably played an important role in the protective immunity against CSFV conferred by the DNA vaccine. In this study, the CMI induced by pSFV1CS-E2 and its derivative pSFV1CS-E2-UL49 encoding a fusion protein of CSFV E2 and pseudorabies virus (PRV) VP22 was evaluated in a mouse model by lymphoproliferation assays based on CFSE or WST-8, intracellular cytokine staining, and cytokine ELISA. The results showed that both vaccines induced CSFV-specific lymphoproliferative responses and cytokine production, and pSFV1CS-E2-UL49 induced stronger lymphoproliferative responses and higher cytokine levels than pSFV1CS-E2. These findings suggest that the alphavirus replicon-delivered DNA vaccines are capable of inducing CMI, and PRV VP22 is able to enhance the immunogenicity of the co-delivered antigen.

分类号: S85

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