1H-NMR revealed pyruvate as a differentially abundant metabolite in the venom glands of Apis cerana and Apis mellifera

文献类型： 外文期刊

第一作者： Zheng, Xing

作者： Zheng, Xing;Liu, Yanjun;Liu, Zhenxing;Zhao, Yazhou;Wang, Rongshen;Geng, Mingyang;Liu, Jinliang;Liu, Zhenxing;Liu, Zhenxing;Zhao, Yazhou

作者机构：

关键词： H-1-NMR; Apis cerana; Apis mellifera; bee venom; metabolomics

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：2.2； 五年影响因子：2.1 ）

ISSN： 0739-4462

年卷期： 2024 年 115 卷 3 期

页码：

收录情况： SCI

摘要： As a common defense mechanism in Hymenoptera, bee venom has complex components. Systematic and comprehensive analysis of bee venom components can aid in early evaluation, accurate diagnosis, and protection of organ function in humans in cases of bee stings. To determine the differences in bee venom composition and metabolic pathways between Apis cerana and Apis mellifera, proton nuclear magnetic resonance (H-1-NMR) technology was used to detect the metabolites in venom samples. A total of 74 metabolites were identified and structurally analyzed in the venom of A. cerana and A. mellifera. Differences in the composition and abundance of major components of bee venom from A. cerana and A. mellifera were mapped to four main metabolic pathways: valine, leucine and isoleucine biosynthesis; glycine, serine and threonine metabolism; alanine, aspartate and glutamate metabolism; and the tricarboxylic acid cycle. These findings indicated that the synthesis and metabolic activities of proteins or polypeptides in bee venom glands were different between A. cerana and A. mellifera. Pyruvate was highly activated in 3 selected metabolic pathways in A. mellifera, being much more dominant in A. mellifera venom than in A. cerana venom. These findings indicated that pyruvate in bee venom glands is involved in various life activities, such as biosynthesis and energy metabolism, by acting as a precursor substance or intermediate product.

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