Proteasomal degradation of MaMYB60 mediated by the E3 ligase MaBAH1 causes high temperature-induced repression of chlorophyll catabolism and green ripening in banana

文献类型： 外文期刊

第一作者： Wei, Wei

作者： Wei, Wei;Yang, Ying-ying;Kuang, Jian-fei;Lu, Wang-jin;Chen, Jian-ye;Shan, Wei;Lakshmanan, Prakash;Lakshmanan, Prakash;Lakshmanan, Prakash;Pang, Xue-qun

作者机构：

期刊名称：PLANT CELL （ 影响因子：11.6； 五年影响因子：12.9 ）

ISSN： 1040-4651

年卷期： 2023 年 35 卷 5 期

页码：

收录情况： SCI

摘要： Temperature-responsive MaBAH1-MaMYB60-CCGs is a dynamic regulatory module that controls chlorophyll catabolism and green ripening in bananas. Banana (Musa acuminata) fruits ripening at 30 degrees C or above fail to develop yellow peels; this phenomenon, called green ripening, greatly reduces their marketability. The regulatory mechanism underpinning high temperature-induced green ripening remains unknown. Here we decoded a transcriptional and post-translational regulatory module that causes green ripening in banana. Banana fruits ripening at 30 degrees C showed greatly reduced expression of 5 chlorophyll catabolic genes (CCGs), MaNYC1 (NONYELLOW COLORING 1), MaPPH (PHEOPHYTINASE), MaTIC55 (TRANSLOCON AT THE INNER ENVELOPE MEMBRANE OF CHLOROPLASTS 55), MaSGR1 (STAY-GREEN 1), and MaSGR2 (STAY-GREEN 2), compared to those ripening at 20 degrees C. We identified a MYB transcription factor, MaMYB60, that activated the expression of all 5 CCGs by directly binding to their promoters during banana ripening at 20 degrees C, while showing a weaker activation at 30 degrees C. At high temperatures, MaMYB60 was degraded. We discovered a RING-type E3 ligase MaBAH1 (benzoic acid hypersensitive 1) that ubiquitinated MaMYB60 during green ripening and targeted it for proteasomal degradation. MaBAH1 thus facilitated MaMYB60 degradation and attenuated MaMYB60-induced transactivation of CCGs and chlorophyll degradation. By contrast, MaMYB60 upregulation increased CCG expression, accelerated chlorophyll degradation, and mitigated green ripening. Collectively, our findings unravel a dynamic, temperature-responsive MaBAH1-MaMYB60-CCG module that regulates chlorophyll catabolism, and the molecular mechanism underpinning green ripening in banana. This study also advances our understanding of plant responses to high-temperature stress.

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