A New Microbial Biosensor for Detecting and Monitoring Water Acute Toxicity Based on Methylene Blue

文献类型： 外文期刊

第一作者： Wu Li-Dong

作者： Wu Li-Dong;Fu Xiao-Chen;Liu Huan;Song Yi;Liu Ling;Li Dan;Zhai Jun-Feng

作者机构：

关键词： Acute water toxicity; Methylene blue; Intact cell; Damaged cell; P. fluorescens

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2016 年 44 卷 9 期

页码：

收录情况： SCI

摘要： A novel method based on methylene blue (MB) was established for the rapid determination of acute water toxicity with the P. fluorescens as the test organism and the damage of cell membrane as the toxicity response. In this method, the damaged rather than intact cells could be stained positively by MB. Therefore, in the presence of toxicants that could induce the damage of cell membrane, MB would enter into cells, resulting in a decrease in MB concentration of the solution. The acute water toxicity could thus be determined by measuring the changes optically in the concentration of MB in the solutions. The present results displayed that, the detection limits of the reported method for 3,5-dichlorophenol, As3+ and Hg2+ were 1. 6, 12. 5 and 3.2 mg/L, respectively. In special, the detection limit of the reported method for 3,5. dichlorophenol (DCP) was lower than that of the commercial equipment, Baroxymeter. The reported method is simple, rapid and reliable, and shows an excellent promise in the determination and monitoring of acute toxicity.

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