Identification of peptides from sinus gland extracts in the red swamp crayfish Procambarus clarkii by mass spectrometry

文献类型： 外文期刊

第一作者： Shui, Yan

作者： Shui, Yan;Xu, Zeng-Hong;Zhou, Xin

作者机构：

关键词： Electrospray ionization-Fourier transform mass spectrometry (ESI-FTMS; Neuropeptides; Procambatrus clarkia; Sinus gland (SG)

期刊名称：INDIAN JOURNAL OF ANIMAL RESEARCH （ 影响因子：0.44； 五年影响因子：0.543 ）

ISSN： 0367-6722

年卷期： 2016 年 50 卷 6 期

页码：

收录情况： SCI

摘要： Crustacean sinus gland (SG) is a well-defined neuroendocrine organ that controls the secretion of various neuropeptides which regulate many physiological activities. The red swamp crayfish Procambarus clarkii is a decapod crustacean with both high economic and scientific importance in China. To facilitate physiological investigations of SG peptide/hormone function in this species, we have been employed tissue extract fractionation by HPLC to obtain a complete description of neuropeptidome and used Electrospray ionization-Fourier transform mass spectrometry (ESI-FTMS) to detect the peptidome present in its organ. In total, 48 peptide sequences belonging to several known neuropeptide families including crustacean cardioactive peptide, CHH precursor-related peptides (CPRPs), orcokinins and pigment dispersing hormones were identified. Among these 48 sequences, 26 are novel peptides and 22 are previously identified. Overall, the results give a stimulus for future physiological studies of SG neuropeptides in P. clarkii and other crustaceans.

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