A novel VIGS method by agroinoculation of cotton seeds and application for elucidating functions of GhBI-1 in salt-stress response

文献类型： 外文期刊

第一作者： Zhang, Jingxia

作者： Zhang, Jingxia;Wang, Furong;Zhang, Chuanyun;Chen, Yu;Liu, Guodong;Zhang, Jun;Hao, Fushun;Zhang, Junhao;Zhao, Yanxiu;Zhang, Jun

作者机构：

关键词： Seed soak agroinoculation VIGS (SSA-VIGS); Cotton; Bax inhibitor-1; Salt tolerance

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2018 年 37 卷 8 期

页码：

收录情况： SCI

摘要： A VIGS method by agroinoculation of cotton seeds was developed for gene silencing in young seedlings and roots, and applied in functional analysis of GhBI-1 in response to salt stress. Virus-induced gene silencing (VIGS) has been widely used to investigate the functions of genes expressed in mature leaves, but not yet in young seedlings or roots of cotton (Gossypium hirsutum L.). Here, we developed a simple and effective VIGS method for silencing genes in young cotton seedlings and roots by soaking naked seeds in Agrobacterium cultures carrying tobacco rattle virus (TRV)-VIGS vectors. When the naked seeds were soaked in Agrobacterium cultures with an OD600 of 1.5 for 90 min, it was optimal for silencing genes effectively in young seedlings as clear photo-bleaching phenotype in the newly emerging leaves of pTRV:GhCLA1 seedlings were observed at 12-14 days post inoculation. Silencing of GhPGF (cotton pigment gland formation) by this method resulted in a 90% decrease in transcript abundances of the gene in roots at the early development stage. We further used the tool to investigate function of GhBI-1 (cotton Bax inhibitor-1) gene in response to salt stress and demonstrated that GhBI-1 might play a protective role under salt stress by suppressing stress-induced cell death in cotton. Our results showed that the newly established VIGS method is a powerful tool for elucidating functions of genes in cotton, especially the genes expressed in young seedlings and roots.

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[1]A novel VIGS method by agroinoculation of cotton seeds and application for elucidating functions of GhBI-1 in salt-stress response. Zhang, Jingxia,Wang, Furong,Zhang, Chuanyun,Chen, Yu,Liu, Guodong,Zhang, Jun,Hao, Fushun,Zhang, Junhao,Zhao, Yanxiu,Zhang, Jun. 2018

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