Targeting trimeric transmembrane domain 5 of oncogenic latent membrane protein 1 using a computationally designed peptide

文献类型： 外文期刊

第一作者： Wang, Yibo

作者： Wang, Yibo;Zhang, Bo;Zhang, Xiaozheng;Li, Hongyuan;Wang, Xiaohui;Wang, Yibo;Peng, Yinghua;Wilson, Andrew J.;Wilson, Andrew J.;Mineev, Konstantin S.;Wang, Xiaohui

作者机构：

期刊名称：CHEMICAL SCIENCE （ 影响因子：9.825； 五年影响因子：9.658 ）

ISSN： 2041-6520

年卷期： 2019 年 10 卷 32 期

页码：

收录情况： SCI

摘要： Protein-protein interactions are involved in diverse biological processes. These interactions are therefore vital targets for drug development. However, the design of peptide modulators targeting membrane-based protein-protein interactions is a challenging goal owing to the lack of experimentally-determined structures and efficient protocols to probe their functions. Here we employed rational peptide design and molecular dynamics simulations to design a membrane-insertable peptide that disrupts the strong trimeric self-association of the fifth transmembrane domain (TMD5) of the oncogenic Epstein-Barr virus (EBV) latent membrane protein-1 (LMP-1). The designed anti-TMD5 peptide formed 1 : 2 heterotrimers with TMD5 in micelles and inhibited TMD5 oligomerization in bacterial membranes. Moreover, the designed peptide inhibited LMP-1 homotrimerization based on NF-kappa B activity in EVB positive lymphoma cells. The results indicated that the designed anti-TMD5 peptide may represent a promising starting point for elaboration of anti-EBV therapeutics via inhibition of LMP-1 oligomerization. To the best of our knowledge, this represents the first example of disrupting homotrimeric transmembrane helices using a designed peptide inhibitor.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序