Analysis of BnMTL, a novel metallothionein-like protein in the bast fiber crop Ramie (Boehmeria nivea)

文献类型： 外文期刊

第一作者： Gao, Gang

作者： Gao, Gang;Chen, Jikang;Chen, Ping;Chen, Kunmei;Abubakar, Aminu Shehu;Yu, Chunming;Zhu, Aiguo

作者机构：

关键词： BnMTL; Cd2+ tolerance; expression pattern; heterologous expression; ramie

期刊名称：FEBS OPEN BIO （ 影响因子：2.693； 五年影响因子：2.519 ）

ISSN： 2211-5463

年卷期： 2019 年 9 卷 9 期

页码：

收录情况： SCI

摘要： Ramie (Boehmeria nivea) is a perennial herb that is highly tolerant of heavy metals. In the present study, we cloned a novel metallothionein-like gene from ramie; this gene, termed BnMTL, encodes a putative 46 amino acid protein with a molecular mass of 4.38 kDa. Analysis using quantitative RT-PCR revealed that cadmium (Cd2+) treatment results in elevated expression of BnMTL in the roots. We heterologously overexpressed BnMTL in Escherichia coli cells to examine its binding to Cd2+ and its possible role in homeostasis. Recombinant E. coli cells expressing BnMTL exhibited a high tolerance of Cd2+ stress up to a concentration of 1 mm, and the observed accumulation of Cd2+ was almost eight-fold higher than the control. These results demonstrate that BnMTL (i) is highly expressed in the root following exposure to Cd2+ and (ii) encodes a typical metallothionein-like protein with high cadmium-binding activity.

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