A selection method of chickens with blue-eggshell and dwarf traits by molecular marker-assisted selection
文献类型: 外文期刊
第一作者: Cui, H. X.
作者: Cui, H. X.;Zheng, M. Q.;Wen, J.;Zhao, G. P.;Shen, Q. C.;Su, Y. C.;Cai, R. C.;Yu, Y.;Chen, Z. W.;Yang, X. R.
作者机构:
关键词: chicken; genetic aggregation; molecular marker-assisted breeding; dwarf; blue-eggshell
期刊名称:POULTRY SCIENCE ( 影响因子:3.352; 五年影响因子:3.679 )
ISSN: 0032-5791
年卷期: 2019 年 98 卷 8 期
页码:
收录情况: SCI
摘要: The blue-eggshell and dwarf traits have an important economic value in poultry production. Using a genetic aggregation-based strategy, the molecular marker-assisted selection technology was jointly used to provide a rapid breeding method for pure strain chickens simultaneously with hens exhibiting the blue eggshell and dwarf traits. Overall, 80 male dwarf chickens and 1,000 hybrid blue-eggshell hens (F0) were used for the hybridization experiment. Subsequently, the crossing of F1 or F2 chicks was performed in succession. The F1 and F2 chicks were respectively detected by the joint molecular markers of the solute carrier organic anion transporter family, namely, 1B3 (SLCO1B3) and the growth hormone receptor (GHR) genes, which relate to blue-eggshell and dwarf traits. Meanwhile, the selection of blue-eggshell and dwarf phenotypes was used to validate the data obtained by the molecular markers. The results showed that F1 chicks included the heterozygous and wild-type of SLCO1B3, as well as the homozygous (hens) and heterozygous (roosters) of GHR. However, F2 chicks included 3 different geno-types of both SLCO1B3 and GHR. Ultimately, 196 F1 roosters (concurrently with heterozygous genotype of SLCO1B3 and GHR) and 1,073 F1 hens (concurrently with heterozygous genotype of SLCO1B3 and homozygous genotype of GHR) were obtained from the initial 10,040 F1 chicks. Further, 27 F2 roosters and 345 F2 hens, which simultaneously carried the homozygous genotype of SLCO1B3 and GHR, were screened from the initial 6,000 F2 chicks. Data obtained on the blue eggshell and dwarf phenotypes were consistent with the results by molecular markers. Similarly, the purity verification of the strain obtained through 2 crossing experiments (F0 male x F2 female and F2 male x F2 female) revealed that all chickens had the blue-eggshell and dwarf traits, supporting that the obtained F2 strain was pure. In summary, for the first time, we successfully bred a pure strain chicken with blue-eggshell and dwarf traits by jointly using the molecular markers of the SLCO1B3 and GHR genes. Our study provides a new method for the rapid cultivation of new chicken strains.
分类号:
- 相关文献
作者其他论文 更多>>
-
Genome-wide association study identifies variants associated with semen volume in white-feathered broilers
作者:Zhang, G. M.;Liu, P. H.;Zheng, J. M.;Zhao, G. P.;Xing, W. H.;Wen, J.;Li, Q. H.;Chen, L.
关键词:GWAS; semen volume; white-feathered broiler
-
Improve the accuracy of chicken leg disease assessment by combination of gait score and X-ray scan
作者:Zheng, J.;Zhang, G.;Ding, J.;He, Z.;Li, Q.;Liu, R.;Wen, J.;Zhao, G.;Liu, D.
关键词:white feather broiler; gait score; X-ray; leg health; growth trait
-
The Importance of Genetic Isolation in Preserving the Gene Pool of Apis cerana in Fanjingshan Nature Reserve
作者:Yu, Y.;Ran, Y.;Zhan, H.;Wan, W.;Wei, X.;Shi, L.;Li, M.
关键词:nature reserve; honey bee resource; protection; genetic differentiation; private haplotype
-
Identification of TPS and TPP gene families in Cannabis sativa and their expression under abiotic stresses
作者:Sun, J.;Xie, D. W.;Sun, J.;Dai, Z. G.;Zhang, X. Y.;Tang, Q.;Cheng, C. H.;Liu, C.;Yu, Y.;Xu, G. C.;Su, J. G.
关键词:Cannabis sativa; cold; drought; phylogenetic tree; salt stress; TPP and TPS gene families; trehalose
-
Genome-Wide Identification and Characterization of the E2F/DP Transcription Factor Family in Triticum aestivum L.
作者:Yu, Y.;Zhang, Y. R.;Song, T. Q.;Zhang, S. X.;Zhang, X. K.;Wang, X. L.;Chen, D. S.
关键词:Triticum aestivum L; E2F; DP; abiotic stress; subcellular localization; self-activation
-
Comparison of amino acid digestibility and its additivity determined with slaughter or cecectomy method for yellow-feather chicken
作者:Liu, H. S.;Zhao, F.;Chen, J.;Yu, Y.;Wang, Y. M.;Sa, R. N.;Xie, J. J.;Zou, Y.;Liu, S. B.;Tan, H. Z.
关键词:additivity; amino acid digestibility; cecectomy method; slaughter method
-
Nuclear and Mitochondrial DNA Suggest That Nature Reserve Maintains Novel Haplotypes and Genetic Diversity of Honeybees (Apis cerana)
作者:Yu, Y.;Zhou, W.;Li, Y.;Wan, W.;Yao, D.;Wei, X.
关键词:anthropogenic activity; genetic conservation; genetic differentiation; genetic diversity; genetic resources