Genome-wide association study of vitamin E using genotyping by sequencing in sesame (Sesamum indicum)

文献类型： 外文期刊

第一作者： He, Qiang

作者： He, Qiang;Xu, Feifei;Min, Myeong-Hyeon;Park, Yong-Jin;He, Qiang;Xu, Feifei;Chu, Sang-Ho;Kim, Kyu-Won;Park, Yong-Jin

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关键词： Genotyping by sequencing; Genome-wide association study; Sesame; Vitamin E

期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）

ISSN： 1976-9571

年卷期： 2019 年 41 卷 9 期

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收录情况： SCI

摘要： Background At least eight structurally related forms of vitamin E occur in nature, four tocopherols and four tocotrienols, all of which are potent membrane-soluble antioxidants. In this study, we detected two major isoforms in sesame (Sesamum indicum L.) seed: gamma-tocopherol and beta-tocotrienol. The objective of this study is to investigate the genetic basis of these vitamin E isoforms. Methods We conducted a genome-wide association study (GWAS) using 5962 genome-wide markers, acquired from 96 core sesame accessions. The GWAS was performed using generalized linear (GLM) and mixed linear (MLM) models. Results LG08_6621957, on chromosome 8, was detected as having a significant association with gamma-tocopherol in both models. It explained 20.9% of gamma-tocopherol variation in sesame. For beta-tocotrienol, no significant loci were detected according to the two models, but one locus, SLG03_13104062, explained 17.8% of the phenotypic variation. Based on structure and phylogenetic studies, the 96 accessions were clearly clustered into two subpopulations. Conclusion This study on sesame demonstrates and provides an evidence that genotyping by sequencing (GBS) based GWAS can be used to identifying important loci for small growing crops. The significant SNPs or genes could be useful for improving the vitamin E content in sesame breeding programs.

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